Anti-Glypican-1 antibody (Internal) (STJ73190)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-Glypican-1 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 60kDa band observed in lysates of cell lines HeLa and K562 (calculated MW of 61.7kDa according to NP_002072.2). Recommended concentration: 1-3µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | GPC1 |
| Gene ID | 2817 |
| Uniprot ID | GPC1_HUMAN |
| Accession Number | NP_002072.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | TDKFWGTSGVES |
Additional Info
| Function | Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination. May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling. |
| Protein Name | Glypican-1 Cleaved Into - Secreted Glypican-1 |
| Database Links | Reactome: R-HSA-1971475Reactome: R-HSA-2022928Reactome: R-HSA-2024096Reactome: R-HSA-202733Reactome: R-HSA-3560783Reactome: R-HSA-3560801Reactome: R-HSA-3656237Reactome: R-HSA-3656253Reactome: R-HSA-376176Reactome: R-HSA-4420332Reactome: R-HSA-9694614Reactome: R-HSA-975634Reactome: R-HSA-9820960Reactome: R-HSA-9833110 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorExtracellular SideEndosomeS-Nitrosylated Form Recycled In EndosomesLocalizes To Cav1-Containing Vesicles Close To The Cell SurfaceCleavage Of Heparan Sulfate Side Chains Takes Place Mainly In Late EndosomesAssociates With Both Forms Of Prnp In Lipid RaftsColocalizes With App In Perinuclear Compartments And With Cp In Intracellular CompartmentsAssociates With Fibrillar App Amyloid-Beta Peptides In Lipid Rafts In Alzheimer Disease BrainsSecreted Glypican-1: SecretedExtracellular Space |
| Alternative Antibody Names | Anti-Glypican-1 Cleaved Into - Secreted Glypican-1 antibodyAnti-GPC1 antibody |
Information sourced from Uniprot.org