Anti-Glutamate Dehydrogenase antibody (Internal) (STJ72157)

SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ72157-100
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General Information

Short DescriptionGoat polyclonal antibody anti-Glutamate Dehydrogenase (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence research applications.
ApplicationsPep-ELISA/WB/IHC/IF
HostGoat
ReactivityHuman/Mouse/Rat/Dog/Cow
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:32000.
WB: Approx 55kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates and in Human, Mouse and Rat Liver lysates (calculated MW of 61.4kDa according to Human NP_005262.1, of 61
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20°C on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolGLUD1
Gene ID2746
Uniprot IDDHE3_HUMAN
Accession NumberNP_005262.1; NP_036216.2
Immunogen RegionInternal
Immunogen SequenceESEEQKRNRVRGILR
SpecificityThis antibody is expected to recognize both reported isoforms (NP_005262.1; NP_036216.2).

Additional Info

Function Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. Plays a role in insulin homeostasis. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.
Protein Name Glutamate Dehydrogenase 1 - Mitochondrial
Gdh 1
Database Links Reactome: R-HSA-2151201
Reactome: R-HSA-8964539
Reactome: R-HSA-9837999
Cellular Localisation Mitochondrion
Endoplasmic Reticulum
Mostly Translocates Into The Mitochondria
Only A Small Amount Of The Protein Localizes To The Endoplasmic Reticulum
Alternative Antibody Names Anti-Glutamate Dehydrogenase 1 - Mitochondrial antibody
Anti-Gdh 1 antibody
Anti-GLUD1 antibody
Anti-GLUD antibody

Information sourced from Uniprot.org

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