Anti-Glutamate Dehydrogenase 1 antibody [R04-9K5] (STJA0037278)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human GLUD1
General Information
| Short Description | Rabbit monoclonal anti-Glutamate Dehydrogenase 1 for use in WB, IHC-P, ICC, IF and FC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ICC/IF/FC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R04-9K5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200FC 1:50-1:100 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | GLUD1 |
| Gene ID | 2746 |
| Uniprot ID | DHE3_HUMAN |
| Immunogen | A synthesized peptide derived from human GLUD1 |
Additional Info
| Post Translational Modifications | ADP-ribosylated by SIRT4, leading to inactivate glutamate dehydrogenase activity. Stoichiometry shows that ADP-ribosylation occurs in one subunit per catalytically active homohexamer. |
| Function | Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. Plays a role in insulin homeostasis. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. |
| Protein Name | Glutamate Dehydrogenase 1 - MitochondrialGdh 1 |
| Database Links | Reactome: R-HSA-2151201Reactome: R-HSA-8964539Reactome: R-HSA-9837999 |
| Cellular Localisation | MitochondrionEndoplasmic ReticulumMostly Translocates Into The MitochondriaOnly A Small Amount Of The Protein Localizes To The Endoplasmic Reticulum |
| Alternative Antibody Names | Anti-Glutamate Dehydrogenase 1 - Mitochondrial antibodyAnti-Gdh 1 antibodyAnti-GLUD1 antibodyAnti-GLUD antibody |
Information sourced from Uniprot.org