• Western blot analysis of extracts of various cell lines, using GLUD2 antibody (STJ28687) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using GLUD2 rabbit polyclonal antibody (STJ28687) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using GLUD2 rabbit polyclonal antibody (STJ28687) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HepG2 cells using GLUD2 rabbit polyclonal antibody (STJ28687) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using GLUD2 rabbit polyclonal antibody (STJ28687) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using GLUD2 rabbit polyclonal antibody (STJ28687) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-GLUD2 antibody (319-558) (STJ28687)

SKU:
STJ28687

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GLUD2 (319-558) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GLUD2
Gene ID: 2747
Uniprot ID: DHE4_HUMAN
Immunogen Region: 319-558
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 319-558 of human GLUD2 (NP_036216.2).
Immunogen Sequence: YLHRFGAKCIAVGESDGSIW NPDGIDPKELEDFKLQHGSI LGFPKAKPYEGSILEVDCDI LIPAATEKQLTKSNAPRVKA KIIAEGANGPTTPEADKIFL ERNILVIPDLYLNAGGVTVS YFEWLKNLNHVSYGRLTFKY ERDSNYHLLLSVQESLERKF GKHGGTIPIVPTAEFQDSIS GASEKDIVHSALAYTMERSA RQIMHTAMKYNLGLDLRTAA YVNAIEKVFKVYSEAGVTF
Tissue Specificity Expressed in retina, testis and, at a lower level, brain.
Post Translational Modifications Stoichiometry shows that ADP-ribosylation occurs in one subunit per catalytically active homohexamer.
Function Important for recycling the chief excitatory neurotransmitter, glutamate, during neurotransmission.
Protein Name Glutamate Dehydrogenase 2 - Mitochondrial
Gdh 2
Database Links Reactome: R-HSA-2151201
Reactome: R-HSA-8964539
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Glutamate Dehydrogenase 2 - Mitochondrial antibody
Anti-Gdh 2 antibody
Anti-GLUD2 antibody
Anti-GLUDP1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance