Anti-GLRX3 antibody (1-335) (STJ28165)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GLRX3 (1-335) antibody for use in WB, IP and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GLRX3 |
| Gene ID | 10539 |
| Uniprot ID | GLRX3_HUMAN |
| Immunogen Region | 1-335 |
| Immunogen Sequence | MAAGAAEAAVAAVEEVGSAG QFEELLRLKAKSLLVVHFWA PWAPQCAQMNEVMAELAKEL PQVSFVKLEAEGVPEVSEKY EISSVPTFLFFKNSQKIDRL DGAHAPELTKKVQRHASSGS FLPSANEHLKEDLNLRLKKL THAAPCMLFMKGTPQEPRCG FSKQMVEILHKHNIQFSSFD IFSDEEVRQGLKAYSSWPTY PQLYVSGELIGGLDIIKELE ASEELDTICPKAPKLEERL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GLRX3 (NP_001186797.1). |
Additional Info
| Tissue Specificity | Expressed in heart, spleen, testis and, to a lower extent, in thymus and peripheral blood leukocytes. Weakly expressed in lung, placenta, colon and small intestine. |
| Function | Together with BOLA2, acts as a cytosolic iron-sulfur (Fe-S) cluster assembly factor that facilitates 2Fe-2S cluster insertion into a subset of cytosolic proteins. Acts as a critical negative regulator of cardiac hypertrophy and a positive inotropic regulator. Required for hemoglobin maturation. Does not possess any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity. |
| Protein Name | Glutaredoxin-3Pkc-Interacting Cousin Of ThioredoxinPicotPkc-Theta-Interacting ProteinPkcq-Interacting ProteinThioredoxin-Like Protein 2 |
| Database Links | Reactome: R-HSA-917937 |
| Cellular Localisation | CytoplasmCytosolCell CortexMyofibrilSarcomereZ LineUnder The Plasma MembraneAfter Pma StimulationGlrx3 And Prkcq/Pkc-Theta Translocate To A More Extended Submembrane AreaIn The Z LineFound Associated With Csrp3 |
| Alternative Antibody Names | Anti-Glutaredoxin-3 antibodyAnti-Pkc-Interacting Cousin Of Thioredoxin antibodyAnti-Picot antibodyAnti-Pkc-Theta-Interacting Protein antibodyAnti-Pkcq-Interacting Protein antibodyAnti-Thioredoxin-Like Protein 2 antibodyAnti-GLRX3 antibodyAnti-PICOT antibodyAnti-TXNL2 antibodyAnti-HUSSY-22 antibody |
Information sourced from Uniprot.org