![Western blot analysis of lysates from wild type (WT) and GLOD4 knockout (KO) HeLa cells, using [KO Validated] GLOD4 Rabbit polyclonal antibody (STJ11100725) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89663/358160/STJ11100725_1__50709.1713122462.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and GLOD4 knockout (KO) HeLa cells, using [KO Validated] GLOD4 Rabbit polyclonal antibody (STJ11100725) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and GLOD4 knockout (KO) HeLa cells, using [KO Validated] GLOD4 Rabbit polyclonal antibody (STJ11100725) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89663/358160/STJ11100725_1__50709.1713122462.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and GLOD4 knockout (KO) HeLa cells, using [KO Validated] GLOD4 Rabbit polyclonal antibody (STJ11100725) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-GLOD4 antibody (179-298) (STJ11100725)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GLOD4 (179-298) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GLOD4 |
| Gene ID | 51031 |
| Uniprot ID | GLOD4_HUMAN |
| Immunogen Region | 179-298 |
| Immunogen Sequence | DNQCKLELQGVKGGVDHAAA FGRIAFSCPQKELPDLEDLM KRENQKILTPLVSLDTPGKA TVQVVILADPDGHEICFVGD EAFRELSKMDPEGSKLLDDA MAADKSDEWFAKHNKPKASG |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 179-298 of human GLOD4 (NP_057164.3). |
Additional Info
| Tissue Specificity | Expressed in heart, brain, liver, kidney, pancreas and placenta. Not expressed in skeletal muscle and lung. |
| Protein Name | Glyoxalase Domain-Containing Protein 4 |
| Database Links | |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Glyoxalase Domain-Containing Protein 4 antibodyAnti-GLOD4 antibodyAnti-C17orf25 antibodyAnti-CGI-150 antibodyAnti-My027 antibody |
Information sourced from Uniprot.org