Western blot analysis of extracts of various cell lines, using GJB1 antibody (STJ112151) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GJB1 antibody (214-283) (STJ112151)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ112151
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General Information

Short DescriptionRabbit polyclonal GJB1 (214-283) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:200-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolGJB1
Gene ID2705
Uniprot IDCXB1_HUMAN
Immunogen Region214-283
Immunogen SequenceIRACARRAQRRSNPPSRKGS GFGHRLSPEYKQNEINKLLS EQDGSLKDILRRSPGTGAGL AEKSDRCSAC
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 214-283 of human GJB1 (NP_000157.1).

Additional Info

Function One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Protein Name Gap Junction Beta-1 Protein
Connexin-32
Cx32
Gap Junction 28 Kda Liver Protein
Database Links Reactome: R-HSA-190704
Reactome: R-HSA-190827
Reactome: R-HSA-190861
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cell Junction
Gap Junction
Alternative Antibody Names Anti-Gap Junction Beta-1 Protein antibody
Anti-Connexin-32 antibody
Anti-Cx32 antibody
Anti-Gap Junction 28 Kda Liver Protein antibody
Anti-GJB1 antibody
Anti-CX32 antibody

Information sourced from Uniprot.org

Citations

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