| Host: |
Mouse |
| Applications: |
WB/ICC |
| Reactivity: |
Chicken/Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Connexin-43 (C-Term) is suitable for use in Western Blot and Immunocytochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
M496 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:1000IHCICC 1:100IP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GJA1 |
| Gene ID: |
2697 |
| Uniprot ID: |
CXA1_HUMAN |
| Immunogen Region: |
C-Term |
| Specificity: |
The antibody detects a 43 kDa* protein corresponding to the apparent molecular mass of connexin-43 on SDS-PAGE immunoblots of mouse brain. The antibody is also useful for immunofluorescent labeling of gap junctions in cells. |
| Immunogen: |
Clone M496 was generated from a peptide fragment in the C-terminal region of human connexin-43. This sequence is highly conserved in mouse and rat connexin-43. |
| Tissue Specificity | Expressed in the heart and fetal cochlea. |
| Post Translational Modifications | Phosphorylated at Ser-368 by PRKCG.phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity. Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly. Phosphorylation at Ser-368 by PRKCD triggers its internalization into small vesicles leading to proteasome-mediated degradation. Sumoylated with SUMO1, SUMO2 and SUMO3, which may regulate the level of functional Cx43 gap junctions at the plasma membrane. May be desumoylated by SENP1 or SENP2. S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication. Acetylated in the developing cortex.leading to delocalization from the cell membrane. |
| Function | Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract. May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles. |
| Protein Name | Gap Junction Alpha-1 ProteinConnexin-43Cx43Gap Junction 43 Kda Heart Protein |
| Database Links | Reactome: R-HSA-190704Reactome: R-HSA-190827Reactome: R-HSA-190840Reactome: R-HSA-190861Reactome: R-HSA-190873Reactome: R-HSA-191650Reactome: R-HSA-196025Reactome: R-HSA-9013406Reactome: R-HSA-9013409Reactome: R-HSA-9705677 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCell JunctionGap JunctionEndoplasmic ReticulumLocalizes At The Intercalated Disk (Icd) In Cardiomyocytes And The Proper Localization At Icd Is Dependent On Tmem65 |
| Alternative Antibody Names | Anti-Gap Junction Alpha-1 Protein antibodyAnti-Connexin-43 antibodyAnti-Cx43 antibodyAnti-Gap Junction 43 Kda Heart Protein antibodyAnti-GJA1 antibodyAnti-GJAL antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
