• Western blot analysis of HepG2 cells using GIT1 Polyclonal Antibody
  • Western blot analysis of the lysates from 293 cells using GIT1 antibody.
  • Western blot analysis of various cells using GIT1 Polyclonal Antibody

Anti-GIT1 antibody (561-610 aa) (STJ93273)

SKU:
STJ93273

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARF GTPase-activating protein GIT1 (561-610 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GIT1
Gene ID: 28964
Uniprot ID: GIT1_HUMAN
Immunogen Region: 561-610 aa
Specificity: GIT1 Polyclonal Antibody detects endogenous levels of GIT1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human GIT1 at the amino acid range 561-610
Function GTPase-activating protein for ADP ribosylation factor family members, including ARF1. Multidomain scaffold protein that interacts with numerous proteins and therefore participates in many cellular functions, including receptor internalization, focal adhesion remodeling, and signaling by both G protein-coupled receptors and tyrosine kinase receptors. Through PAK1 activation, positively regulates microtubule nucleation during interphase. Plays a role in the regulation of cytokinesis.for this function, may act in a pathway also involving ENTR1 and PTPN13. May promote cell motility both by regulating focal complex dynamics and by local activation of RAC1. May act as scaffold for MAPK1/3 signal transduction in focal adhesions. Recruits MAPK1/3/ERK1/2 to focal adhesions after EGF stimulation via a Src-dependent pathway, hence stimulating cell migration. Plays a role in brain development and function. Involved in the regulation of spine density and synaptic plasticity that is required for processes involved in learning. Plays an important role in dendritic spine morphogenesis and synapse formation. In hippocampal neurons, recruits guanine nucleotide exchange factors (GEFs), such as ARHGEF7/beta-PIX, to the synaptic membrane. These in turn locally activate RAC1, which is an essential step for spine morphogenesis and synapse formation. May contribute to the organization of presynaptic active zones through oligomerization and formation of a Piccolo/PCLO-based protein network, which includes ARHGEF7/beta-PIX and FAK1. In neurons, through its interaction with liprin-alpha family members, may be required for AMPA receptor (GRIA2/3) proper targeting to the cell membrane. In complex with GABA(A) receptors and ARHGEF7, plays a crucial role in regulating GABA(A) receptor synaptic stability, maintaining GPHN/gephyrin scaffolds and hence GABAergic inhibitory synaptic transmission, by locally coordinating RAC1 and PAK1 downstream effector activity, leading to F-actin stabilization. May also be important for RAC1 downstream signaling pathway through PAK3 and regulation of neuronal inhibitory transmission at presynaptic input. Required for successful bone regeneration during fracture healing. The function in intramembranous ossification may, at least partly, exerted by macrophages in which GIT1 is a key negative regulator of redox homeostasis, IL1B production, and glycolysis, acting through the ERK1/2/NRF2/NFE2L2 axis. May play a role in angiogenesis during fracture healing. In this process, may regulate activation of the canonical NF-kappa-B signal in bone mesenchymal stem cells by enhancing the interaction between NEMO and 'Lys-63'-ubiquitinated RIPK1/RIP1, eventually leading to enhanced production of VEGFA and others angiogenic factors. Essential for VEGF signaling through the activation of phospholipase C-gamma and ERK1/2, hence may control endothelial cell proliferation and angiogenesis.
Protein Name Arf Gtpase-Activating Protein Git1
Arf Gap Git1
Cool-Associated And Tyrosine-Phosphorylated Protein 1
Cat-1
Cat1
G Protein-Coupled Receptor Kinase-Interactor 1
Grk-Interacting Protein 1
P95-App1
Database Links Reactome: R-HSA-3928664
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013406
Reactome: R-HSA-9013409
Reactome: R-HSA-9013420
Reactome: R-HSA-9013423
Reactome: R-HSA-9013424
Reactome: R-HSA-9619229
Cellular Localisation Cytoplasm
Synapse
Presynapse
Postsynapse
Postsynaptic Density
Cell Junction
Focal Adhesion
Cell Projection
Lamellipodium
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle Pole
Cycles Between At Least 3 Distinct Intracellular Compartments
Including Focal Adhesions
Cytosolic Complexes
Containing At Least Pxn/Paxillin
Arhgef7 And Pak1
And Membrane Protrusions
During Cell Migration
Moves From The Disassembling Adhesions Into The Cytosol And Towards The Leading Edge
In Adherent Cells
Localizes To Adhesions
Recruitment To Adhesions May Be Mediated By Rac And Active Tyrosine-Phosphorylated Pxn
May Be Present In Both Excitatory And Inhibitory Synapses
In Hippocampal Neurons
Recruitment Of Git1 To Synapses Is Regulated By Ephrinb Activation And Ephrinb Downstream Effector Grb4/Nck2
Partially Colocalizes With Pclo
Interaction With Grin3a Limits Git1 Synaptic Localization
Localization To The Centrosome Does Not Depend Upon The Presence Of Gamma-Tubulin
Alternative Antibody Names Anti-Arf Gtpase-Activating Protein Git1 antibody
Anti-Arf Gap Git1 antibody
Anti-Cool-Associated And Tyrosine-Phosphorylated Protein 1 antibody
Anti-Cat-1 antibody
Anti-Cat1 antibody
Anti-G Protein-Coupled Receptor Kinase-Interactor 1 antibody
Anti-Grk-Interacting Protein 1 antibody
Anti-P95-App1 antibody
Anti-GIT1 antibody

Information sourced from Uniprot.org

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