Anti-Ghrelin antibody (24-51 aa) (STJA0040723)
SPECIFICATIONS
ClonalityPolyclonal
HostGuinea Pig
ConjugationUnconjugated
IsotypeMixed
ImmunogenA synthetic peptide (GSSFLSPEHQRVQQRKESKKPPAKLQPR) corresponding to the amino acids 24-51 from human ghrelin. This peptide was conjugated to carrier protein to enhance the immunological response.
General Information
| Short Description | Guinea Pig polyclonal anti-Ghrelin (24-51 aa) for use in IHC-F in Human and Sheep samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-F |
| Host | Guinea Pig |
| Reactivity | Human/Sheep |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | Mixed |
| Conjugation | Unconjugated |
| Purification | Whole serum |
| Dilution Range | IHC 2.125 |
| Storage Instruction | Spin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C or lower. After reconstitution, divide into aliquots and store at-20°C, or low |
Target Information
| Gene Symbol | GHRL |
| Gene ID | 51738 |
| Uniprot ID | GHRL_HUMAN |
| Immunogen | A synthetic peptide (GSSFLSPEHQRVQQRKESKKPPAKLQPR) corresponding to the amino acids 24-51 from human ghrelin. This peptide was conjugated to carrier protein to enhance the immunological response. |
| Immunogen Region | 24-51 aa |
| Immunogen Sequence | Human |
| Specificity | Specificity was demonstrated by immunohistochemistry. This antibody is known to react with sheep and human. Other species have not yet been tested. |
Additional Info
| Tissue Specificity | Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy. |
| Post Translational Modifications | O-octanoylated by GOAT/MBOAT4. O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid. Also O-acetylated and O-butyrylated on Ser-26 to minor levels. Amidation of Leu-98 is essential for obestatin activity. |
| Function | Ghrelin-27: Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation. Ghrelin-28: Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation. Obestatin: May be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility. |
| Protein Name | Appetite-Regulating HormoneGrowth Hormone SecretagogueGrowth Hormone-Releasing PeptideMotilin-Related PeptideProtein M46 Cleaved Into - Ghrelin-27 - Ghrelin-28Ghrelin - Obestatin |
| Database Links | Reactome: R-HSA-375276 Q9UBU3-1Reactome: R-HSA-416476 Q9UBU3-1Reactome: R-HSA-422085 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Appetite-Regulating Hormone antibodyAnti-Growth Hormone Secretagogue antibodyAnti-Growth Hormone-Releasing Peptide antibodyAnti-Motilin-Related Peptide antibodyAnti-Protein M46 Cleaved Into - Ghrelin-27 - Ghrelin-28 antibodyAnti-Ghrelin - Obestatin antibodyAnti-GHRL antibodyAnti-MTLRP antibodyAnti-UNQ524 antibodyAnti-PRO1066 antibody |
Information sourced from Uniprot.org