• STJ72966 (0. 1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-GGT1 antibody (180-193) (STJ72966)

SKU:
STJ72966-100

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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-GGT1 (180-193) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.1-0.3µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: GGT1
Gene ID: 2678
Uniprot ID: GGT1_HUMAN
Immunogen Region: 180-193
Accession Number: NP_005256.2
Specificity: Reported variants represent identical protein: NP_038347.2, NP_001027536.1, NP_001027537.1, NP_005256.2.
Immunogen Sequence: NKRTVIEQQPVLCE
Post Translational Modifications N-glycosylated on both chains. Contains hexoses, hexosamines and sialic acid residues. Glycosylation profiles tested in kidney and liver tissues reveal the presence of tissue-specific and site-specific glycan composition, despite the overlap in composition among the N-glycans. A total of 36 glycan compositions, with 40 unique structures are observed. Up to 15 different glycans are observed at a single site, with site-specific variation in glycan composition. The difference in glycosylation profiles in the 2 tissues do not affect the enzyme activity. Cleaved by autocatalysis into a large and a small subunit and the autocatalytic cleavage is essential to the functional activation of the enzyme.
Function Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates (such as maresin conjugate (13R)-S-glutathionyl-(14S)-hydroxy-(4Z,7Z,9E,11E,16Z,19Z)-docosahexaenoate, MCTR1) and other gamma-glutamyl compounds (such as leukotriene C4, LTC4). The metabolism of glutathione by GGT1 releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Contributes to cysteine homeostasis, glutathione homeostasis and in the conversion of the leukotriene LTC4 to LTD4. Isoform 3: Seems to be inactive.
Protein Name Glutathione Hydrolase 1 Proenzyme
Gamma-Glutamyltransferase 1
Gamma-Glutamyltranspeptidase 1
Ggt 1
Leukotriene-C4 Hydrolase
Cd Antigen Cd224 Cleaved Into - Glutathione Hydrolase 1 Heavy Chain - Glutathione Hydrolase 1 Light Chain
Database Links Reactome: R-HSA-174403
Reactome: R-HSA-2142691
Reactome: R-HSA-5423646
Reactome: R-HSA-5579022
Reactome: R-HSA-9035968
Reactome: R-HSA-9664535
Reactome: R-HSA-9753281
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Glutathione Hydrolase 1 Proenzyme antibody
Anti-Gamma-Glutamyltransferase 1 antibody
Anti-Gamma-Glutamyltranspeptidase 1 antibody
Anti-Ggt 1 antibody
Anti-Leukotriene-C4 Hydrolase antibody
Anti-Cd Antigen Cd224 Cleaved Into - Glutathione Hydrolase 1 Heavy Chain - Glutathione Hydrolase 1 Light Chain antibody
Anti-GGT1 antibody
Anti-GGT antibody

Information sourced from Uniprot.org

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