Anti-GFP-Tag antibody (1-238 aa) [SRM5521] {Affinity Gel} (STJ11107594)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationAffinity Gel
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal GFP-Tag (1-238 aa) antibody for use in IP in species independent samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IP |
| Host | Rabbit |
| Reactivity | Species Independent |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM5521 |
| Isotype | IgG |
| Conjugation | Affinity Gel |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | IP:20 Mu l-40 Mu l Affinity Gel for 100 Mu g-300 Mu g extracts of whole cells |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Immunogen Region | 1-238 aa |
| Immunogen Sequence | MSKGEELFTGVVPILVELDG DVNGHKFSVSGEGEGDATYG KLTLKFICTTGKLPVPWPTL VTTLTYGVQCFSRYPDHMKR HDFFKSAMPEGYVQERTISF KDDGNYKTRAEVKFEGDTLV NRIELKGIDFKEDGNILGHK LEYNYNSHNVYITADKQKNG IKANFKTRHNIEDGGVQLAD HYQQNTPIGDGPVLLPDNHY LSTQSALSKDPNEKRDHMVL LEFVTAAGITHGMDELYK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-238 of GFP. |
Additional Info
| Background | The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes/substrates other than molecular oxygen.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. |
Information sourced from Uniprot.org