• Western blot analysis of various lysates, using GFM1 rabbit polyclonal antibody (STJ23773) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using GFM1 antibody (STJ23773) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using GFM1 antibody (STJ23773) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-GFM1 antibody (482-751) (STJ23773)

SKU:
STJ23773

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GFM1 (482-751) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GFM1
Gene ID: 85476
Uniprot ID: EFGM_HUMAN
Immunogen Region: 482-751
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 482-751 of human GFM1 (NP_079272.4).
Immunogen Sequence: VYFDTENKETVISGMGELHL EIYAQRLEREYGCPCITGKP KVAFRETITAPVPFDFTHKK QSGGAGQYGKVIGVLEPLDP EDYTKLEFSDETFGSNIPKQ FVPAVEKGFLDACEKGPLSG HKLSGLRFVLQDGAHHMVDS NEISFIRAGEGALKQALANA TLCILEPIMAVEVVAPNEFQ GQVIAGINRRHGVITGQDGV EDYFTLYADVPLNDMFGYST ELRSCTEGKGEYTMEYSRY
Function Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Does not mediate the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis.
Protein Name Elongation Factor G - Mitochondrial
Ef-Gmt
Elongation Factor G 1 - Mitochondrial
Mef-G 1
Elongation Factor G1
Hefg1
Database Links Reactome: R-HSA-5389840
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Elongation Factor G - Mitochondrial antibody
Anti-Ef-Gmt antibody
Anti-Elongation Factor G 1 - Mitochondrial antibody
Anti-Mef-G 1 antibody
Anti-Elongation Factor G1 antibody
Anti-Hefg1 antibody
Anti-GFM1 antibody
Anti-EFG antibody
Anti-EFG1 antibody
Anti-GFM antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance