• Western blot analysis of various lysates using GEMIN6 Rabbit polyclonal antibody (STJ114253) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GEMIN6 antibody (1-167) (STJ114253)

SKU:
STJ114253

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GEMIN6 (1-167) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GEMIN6
Gene ID: 79833
Uniprot ID: GEMI6_HUMAN
Immunogen Region: 1-167
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-167 of human GEMIN6 (NP_079051.9).
Immunogen Sequence: MSEWMKKGPLEWQDYIYKEV RVTASEKNEYKGWVLTTDPV SANIVLVNFLEDGSMSVTGI MGHAVQTVETMNEGDHRVRE KLMHLFTSGDCKAYSPEDLE ERKNSLKKWLEKNHIPITEQ GDAPRTLCVAGVLTIDPPYG PENCSSSNEIILSRVQDLIE GHLTASQ
Function The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Binding of snRNA inside 5Sm triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP.
Protein Name Gem-Associated Protein 6
Gemin-6
Sip2
Database Links Reactome: R-HSA-191859
Reactome: R-HSA-9754678
Cellular Localisation Nucleus
Nucleoplasm
Gem
Cytoplasm
Found Both In The Nucleoplasm And In Nuclear Bodies Called Gems (Gemini Of Cajal Bodies) That Are Often In Proximity To Cajal (Coiled) Bodies
Also Found In The Cytoplasm
Alternative Antibody Names Anti-Gem-Associated Protein 6 antibody
Anti-Gemin-6 antibody
Anti-Sip2 antibody
Anti-GEMIN6 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance