| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | GEMIN2 |
| Gene ID: | 8487 |
| Uniprot ID: | GEMI2_HUMAN |
| Immunogen Region: | 180-280 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 180-280 of human Gemin 2 (NP_003607.1) |
| Immunogen Sequence: | LSIVSRMNQATVTSVLEYLS NWFGERDFTPELGRWLYALL ACLEKPLLPEAHSLIRQLAR RCSEVRLLVDSKDDERVPAL NLLICLVSRYFDQRDLADEP S |
| Function | The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG (5Sm) are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A. Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. Within the SMN complex, GEMIN2 constrains the conformation of 5Sm, thereby promoting 5Sm binding to snRNA containing the snRNP code (a nonameric Sm site and a 3'-adjacent stem-loop), thus preventing progression of assembly until a cognate substrate is bound. |
| Protein Name | Gem-Associated Protein 2 Gemin-2 Component Of Gems 2 Survival Of Motor Neuron Protein-Interacting Protein 1 Smn-Interacting Protein 1 |
| Database Links | Reactome: R-HSA-191859 Reactome: R-HSA-9754678 |
| Cellular Localisation | Nucleus Gem Cytoplasm Localized In Subnuclear Structures Next To Coiled Bodies Called Gems Which Are Highly Enriched In Spliceosomal Snrnps Also Found In The Cytoplasm |
| Alternative Antibody Names | Anti-Gem-Associated Protein 2 antibody Anti-Gemin-2 antibody Anti-Component Of Gems 2 antibody Anti-Survival Of Motor Neuron Protein-Interacting Protein 1 antibody Anti-Smn-Interacting Protein 1 antibody Anti-GEMIN2 antibody Anti-SIP1 antibody |
Information sourced from Uniprot.org