Anti-GEF5 antibody (Internal) (STJ70864)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-GEF5 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 170kDa band observed in Human HeLa lysates (calculated MW of 177kDa according to NP_005426.2). Recommended concentration: 0.1-0.3Β΅g/ml.IHC: Paraffin embedded Human Placenta |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20Β°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | ARHGEF5 |
| Gene ID | 7984 |
| Uniprot ID | ARHG5_HUMAN |
| Accession Number | NP_005426.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | DKEIDQNSQQEE |
Additional Info
| Post Translational Modifications | Activation of SRC induces tyrosine phosphorylation of ARHGEF5. |
| Function | Guanine nucleotide exchange factor which activates Rho GTPases. Strongly activates RHOA. Also strongly activates RHOB, weakly activates RHOC and RHOG and shows no effect on RHOD, RHOV, RHOQ or RAC1. Involved in regulation of cell shape and actin cytoskeletal organization. Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia. Required for SRC-induced podosome formation. Involved in positive regulation of immature dendritic cell migration. |
| Protein Name | Rho Guanine Nucleotide Exchange Factor 5Ephexin-3Guanine Nucleotide Regulatory Protein TimOncogene TimTransforming Immortalized Mammary OncogeneP60 Tim |
| Database Links | Reactome: R-HSA-193648Reactome: R-HSA-416482Reactome: R-HSA-8980692Reactome: R-HSA-9013026Reactome: R-HSA-9013106Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013408 |
| Cellular Localisation | CytoplasmNucleusCell ProjectionPodosome |
| Alternative Antibody Names | Anti-Rho Guanine Nucleotide Exchange Factor 5 antibodyAnti-Ephexin-3 antibodyAnti-Guanine Nucleotide Regulatory Protein Tim antibodyAnti-Oncogene Tim antibodyAnti-Transforming Immortalized Mammary Oncogene antibodyAnti-P60 Tim antibodyAnti-ARHGEF5 antibodyAnti-TIM antibody |
Information sourced from Uniprot.org