Anti-GDF9 antibody (205-454) (STJ23768)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GDF9 (205-454) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GDF9 |
| Gene ID | 2661 |
| Uniprot ID | GDF9_HUMAN |
| Immunogen Region | 205-454 |
| Immunogen Sequence | FGKKHKWIQIDVTSLLQPLV ASNKRSIHMSINFTCMKDQL EHPSAQNGLFNMTLVSPSLI LYLNDTSAQAYHSWYSLHYK RRPSQGPDQERSLSAYPVGE EAAEDGRSSHHRHRRGQETV SSELKKPLGPASFNLSEYFR QFLLPQNECELHDFRLSFSQ LKWDNWIVAPHRYNPRYCKG DCPRAVGHRYGSPVHTMVQN IIYEKLDSSVPRPSCVPAKY SPLSVLTIEPDGSIAYKEY |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 205-454 of human GDF9 (NP_005251.1). |
Additional Info
| Tissue Specificity | Expressed in ovarian granulosa cells. Present in oocytes of primary follicles (at protein level). |
| Post Translational Modifications | Phosphorylated.phosphorylation is critical for GDF9 function. In vitro, can be phosphorylated by CK at Ser-325. |
| Function | Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells. |
| Protein Name | Growth/Differentiation Factor 9Gdf-9 |
| Database Links | |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Growth/Differentiation Factor 9 antibodyAnti-Gdf-9 antibodyAnti-GDF9 antibody |
Information sourced from Uniprot.org