| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:5000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | GDF9 |
| Gene ID: | 2661 |
| Uniprot ID: | GDF9_HUMAN |
| Immunogen Region: | 205-454 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 205-454 of human GDF9 (NP_005251.1). |
| Immunogen Sequence: | FGKKHKWIQIDVTSLLQPLV ASNKRSIHMSINFTCMKDQL EHPSAQNGLFNMTLVSPSLI LYLNDTSAQAYHSWYSLHYK RRPSQGPDQERSLSAYPVGE EAAEDGRSSHHRHRRGQETV SSELKKPLGPASFNLSEYFR QFLLPQNECELHDFRLSFSQ LKWDNWIVAPHRYNPRYCKG DCPRAVGHRYGSPVHTMVQN IIYEKLDSSVPRPSCVPAKY SPLSVLTIEPDGSIAYKEY |
| Tissue Specificity | Expressed in ovarian granulosa cells. Present in oocytes of primary follicles (at protein level). |
| Post Translational Modifications | Phosphorylated.phosphorylation is critical for GDF9 function. In vitro, can be phosphorylated by CK at Ser-325. |
| Function | Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells. |
| Protein Name | Growth/Differentiation Factor 9Gdf-9 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Growth/Differentiation Factor 9 antibodyAnti-Gdf-9 antibodyAnti-GDF9 antibody |
Information sourced from Uniprot.org