• Immunohistochemistry analysis of paraffin-embedded rat kidney using GCLC Rabbit monoclonal antibody (STJ11102089) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using GCLC Rabbit monoclonal antibody (STJ11102089) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Western blot analysis of extracts of Rat kidney, using GCLC Rabbit monoclonal antibody (STJ11102089) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-GCLC antibody (538-637) [S9MR] (STJ11102089)

SKU:
STJ11102089

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-GCLC (538-637) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GCLC
Gene ID: 2729
Uniprot ID: GSH1_HUMAN
Immunogen Region: 538-637
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 538-637 of human GCLC (P48506).
Immunogen Sequence: LNSYLENMEVDVDTRCSILN YLKLIKKRASGELMTVARWM REFIANHPDYKQDSVITDEM NYSLILKCNQIANELCECPE LLGSAFRKVKYSGSKTDSSN
Function Catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine and participates in the first and rate-limiting step in glutathione biosynthesis.
Protein Name Glutamate--Cysteine Ligase Catalytic Subunit
Gcs Heavy Chain
Gamma-Ecs
Gamma-Glutamylcysteine Synthetase
Database Links Reactome: R-HSA-174403
Reactome: R-HSA-5578999
Reactome: R-HSA-9818027
Alternative Antibody Names Anti-Glutamate--Cysteine Ligase Catalytic Subunit antibody
Anti-Gcs Heavy Chain antibody
Anti-Gamma-Ecs antibody
Anti-Gamma-Glutamylcysteine Synthetase antibody
Anti-GCLC antibody
Anti-GLCL antibody
Anti-GLCLC antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance