Anti-GCH1 antibody (Internal) (STJ71476)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-GCH1 (Internal) is suitable for use in ELISA and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:16000.IHC: Paraffin embedded Human Brain (Cerebellum) and Small Intestine. Recommended concentration: 5µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | GCH1 |
| Gene ID | 2643 |
| Uniprot ID | GCH1_HUMAN |
| Accession Number | NP_000152.1 ; NP_001019195.1 ; NP_001019241.1 ; NP_001019242.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | GKVHIGYLPNKQ |
| Specificity | This antibody is expected to recognize all four isoforms (NP_000152.1 ; NP_001019195.1 ; NP_001019241.1 ; NP_001019242.1) |
Additional Info
| Post Translational Modifications | Phosphorylated by casein kinase II at Ser-81 in HAECs during oscillatory shear stress.phosphorylation at Ser-81 results in increased enzyme activity. |
| Function | Positively regulates nitric oxide synthesis in umbilical vein endothelial cells (HUVECs). May be involved in dopamine synthesis. May modify pain sensitivity and persistence. Isoform GCH-1 is the functional enzyme, the potential function of the enzymatically inactive isoforms remains unknown. |
| Protein Name | Gtp Cyclohydrolase 1Gtp Cyclohydrolase IGtp-Ch-I |
| Database Links | Reactome: R-HSA-1474151 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Gtp Cyclohydrolase 1 antibodyAnti-Gtp Cyclohydrolase I antibodyAnti-Gtp-Ch-I antibodyAnti-GCH1 antibodyAnti-DYT5 antibodyAnti-GCH antibody |
Information sourced from Uniprot.org