• Confocal imaging of paraffin-embedded Mouse testis tissue using GATA4 Rabbit polyclonal antibody (STJ115704, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Human testis tissue using GATA4 Rabbit polyclonal antibody (STJ115704, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of Hep G2 cells using GATA4 Rabbit polyclonal antibody (STJ115704, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry analysis of GATA4 in paraffin-embedded rat spleen using GATA4 Rabbit polyclonal antibody (STJ115704) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of GATA4 in paraffin-embedded mouse lung using GATA4 Rabbit polyclonal antibody (STJ115704) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of GATA4 in paraffin-embedded human colon carcinoma using GATA4 Rabbit polyclonal antibody (STJ115704) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Rat stomach, using GATA4 Rabbit polyclonal antibody (STJ115704) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of various lysates using GATA4 Rabbit polyclonal antibody (STJ115704) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-GATA4 antibody (350-442) (STJ115704)

SKU:
STJ115704

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GATA4 (350-442) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GATA4
Gene ID: 2626
Uniprot ID: GATA4_HUMAN
Immunogen Region: 350-442
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 350-442 of human GATA4 (NP_002043.2).
Immunogen Sequence: SSNATTSSSEEMRPIKTEPG LSSHYGHSSSVSQTFSVSAM SGHGPSIHPVLSALKLSPQG YASPVSQSPQTSSKQDSWNS LVLADSHGDIITA
Post Translational Modifications Methylation at Lys-300 attenuates transcriptional activity.
Function Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function. In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it down-regulates endocardial and endothelial gene expression. Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions. Acts as a transcriptional activator of ANF in cooperation with NKX2-5. Promotes cardiac myocyte enlargement. Required during testicular development. May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, sphingosine-1-phosphate lyase.
Protein Name Transcription Factor Gata-4
Gata-Binding Factor 4
Database Links Reactome: R-HSA-2032785
Reactome: R-HSA-400511
Reactome: R-HSA-5578768
Reactome: R-HSA-9690406
Reactome: R-HSA-9733709
Reactome: R-HSA-9758920
Reactome: R-HSA-9823730
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor Gata-4 antibody
Anti-Gata-Binding Factor 4 antibody
Anti-GATA4 antibody

Information sourced from Uniprot.org

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