Anti-GATA4 antibody (343-442) [S9MR] (STJ11102039)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal GATA4 (343-442) antibody for use in WB, IHC-P and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S9MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000IHC-P:1:200-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GATA4 |
| Gene ID | 2626 |
| Uniprot ID | GATA4_HUMAN |
| Immunogen Region | 343-442 |
| Immunogen Sequence | ASGASSNSSNATTSSSEEMR PIKTEPGLSSHYGHSSSVSQ TFSVSAMSGHGPSIHPVLSA LKLSPQGYASPVSQSPQTSS KQDSWNSLVLADSHGDIITA |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 343-442 of human GATA4 (NP_002043.2). |
Additional Info
| Post Translational Modifications | Methylation at Lys-300 attenuates transcriptional activity. |
| Function | Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function. In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it down-regulates endocardial and endothelial gene expression. Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions. Acts as a transcriptional activator of ANF in cooperation with NKX2-5. Promotes cardiac myocyte enlargement. Required during testicular development. May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, sphingosine-1-phosphate lyase. |
| Protein Name | Transcription Factor Gata-4Gata-Binding Factor 4 |
| Database Links | Reactome: R-HSA-2032785Reactome: R-HSA-400511Reactome: R-HSA-5578768Reactome: R-HSA-9690406Reactome: R-HSA-9733709Reactome: R-HSA-9758920Reactome: R-HSA-9823730Reactome: R-HSA-983231Reactome: R-HSA-9925561 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Factor Gata-4 antibodyAnti-Gata-Binding Factor 4 antibodyAnti-GATA4 antibody |
Information sourced from Uniprot.org