Anti-GATA4 antibody (228-277 aa) (STJ93227)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human GATA4 at the amino acid range 228-277
General Information
| Short Description | Rabbit polyclonal anti-Transcription factor GATA-4 (228-277 aa) for use in WB, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IF 1:200-1:1000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GATA4 |
| Gene ID | 2626 |
| Uniprot ID | GATA4_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human GATA4 at the amino acid range 228-277 |
| Immunogen Region | 228-277 aa |
| Specificity | GATA-4 Polyclonal Antibody detects endogenous levels of GATA-4 protein. |
Additional Info
| Function | Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function. In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it down-regulates endocardial and endothelial gene expression. Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions. Acts as a transcriptional activator of ANF in cooperation with NKX2-5. Promotes cardiac myocyte enlargement. Required during testicular development. May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, sphingosine-1-phosphate lyase. |
| Protein Name | Transcription Factor Gata-4Gata-Binding Factor 4 |
| Database Links | Reactome: R-HSA-2032785Reactome: R-HSA-400511Reactome: R-HSA-5578768Reactome: R-HSA-9690406Reactome: R-HSA-9733709Reactome: R-HSA-9758920Reactome: R-HSA-9823730Reactome: R-HSA-983231Reactome: R-HSA-9925561 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Factor Gata-4 antibodyAnti-Gata-Binding Factor 4 antibodyAnti-GATA4 antibody |
Information sourced from Uniprot.org