Anti-GATA3 antibody (1-444) (STJ28052)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GATA3 (1-444) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GATA3 |
| Gene ID | 2625 |
| Uniprot ID | GATA3_HUMAN |
| Immunogen Region | 1-444 |
| Immunogen Sequence | MEVTADQPRWVSHHHPAVLN GQHPDTHHPGLSHSYMDAAQ YPLPEEVDVLFNIDGQGNHV PPYYGNSVRATVQRYPPTHH GSQVCRPPLLHGSLPWLDGG KALGSHHTASPWNLSPFSKT SIHHGSPGPLSVYPPASSSS LSGGHASPHLFTFPPTPPKD VSPDPSLSTPGSAGSARQDE KECLKYQVPLPDSMKLESSH SRGSMTALGGASSSTHHPIT TYPPYVPEYSSGLFPPSSL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human GATA3 (NP_001002295.1). |
Additional Info
| Tissue Specificity | T-cells and endothelial cells. |
| Function | Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression. Coordinates macrophage transcriptional activation and UCP2-dependent metabolic reprogramming in response to IL33. Upon tissue injury, acts downstream of IL33 signaling to drive differentiation of inflammation-resolving alternatively activated macrophages. |
| Protein Name | Trans-Acting T-Cell-Specific Transcription Factor Gata-3Gata-Binding Factor 3 |
| Database Links | Reactome: R-HSA-5689880Reactome: R-HSA-6785807Reactome: R-HSA-8939236Reactome: R-HSA-9018519Reactome: R-HSA-9830364Reactome: R-HSA-983231 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Trans-Acting T-Cell-Specific Transcription Factor Gata-3 antibodyAnti-Gata-Binding Factor 3 antibodyAnti-GATA3 antibody |
Information sourced from Uniprot.org