• Western blot analysis of extracts of various cell lines, using GATA2 Rabbit monoclonal antibody (STJ11102246) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Western blot analysis of extracts from wild type (WT) and GATA2 knockout (KO) HeLa cells, using GATA2 antibody (STJ11102246) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-GATA2 antibody (1-210) [S6MR] (STJ11102246)

SKU:
STJ11102246

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-GATA2/3 (1-210) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-210
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human GATA2/3 (NP_116027.2).
Immunogen Sequence: MEVAPEQPRWMAHPAVLNAQ HPDSHHPGLAHNYMEPAQLL PPDEVDVFFNHLDSQGNPYY ANPAHARARVSYSPAHARLT GGQMCRPHLLHSPGLPWLDG GKAALSAAAAHHHNPWTVSP FSKTPLHPSAAGGPGGPLSV YPGAGGGSGGGSGSSVASLT PTAAHSGSHLFGFPPTPPKE VSPDPSTTGAASPASSSAGG SAARGEDKDG
Background This gene encodes a protein that shares high sequence similarity with ribosomal protein L26. Alternative splicing results in multiple transcript variants encoding the same protein.

Information sourced from Uniprot.org

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