• Western blot analysis of extracts of various cell lines, using GATA2 Rabbit monoclonal antibody (STJ11102246) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Western blot analysis of extracts from wild type (WT) and GATA2 knockout (KO) HeLa cells, using GATA2 antibody (STJ11102246) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-GATA2 antibody (1-210) [S6MR] (STJ11102246)

SKU:
STJ11102246

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GATA2
GATA3
Gene ID: 2624
2625
Uniprot ID: GATA2_HUMAN
GATA3_HUMAN
Immunogen Region: 1-210
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human GATA2/3 (NP_116027.2).
Immunogen Sequence: MEVAPEQPRWMAHPAVLNAQ HPDSHHPGLAHNYMEPAQLL PPDEVDVFFNHLDSQGNPYY ANPAHARARVSYSPAHARLT GGQMCRPHLLHSPGLPWLDG GKAALSAAAAHHHNPWTVSP FSKTPLHPSAAGGPGGPLSV YPGAGGGSGGGSGSSVASLT PTAAHSGSHLFGFPPTPPKE VSPDPSTTGAASPASSSAGG SAARGEDKDG
Tissue Specificity P23769 - Endothelial cells.
P23771 - T-cells and endothelial cells.
Alternative Antibody Names Anti-Endothelial Transcription Factor Gata-2 antibody
Anti-Gata-Binding Protein 2 antibody
Anti-GATA2 antibody
Anti-Trans-Acting T-Cell-Specific Transcription Factor Gata-3 antibody
Anti-Gata-Binding Factor 3 antibody
Anti-GATA3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance