Anti-GATA1 antibody [SRM14459] (STJ11109189)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide. This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit monoclonal GATA1 antibody for use in WB, IP and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM14459 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB, 1:4000-1:16000IP, 0.5 Mu g-4 Mu g antibody for 200 Mu g-600 Mu g extracts of whole cellsELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GATA1 |
| Gene ID | 2623 |
| Uniprot ID | GATA1_HUMAN |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
Additional Info
| Tissue Specificity | Erythrocytes. |
| Post Translational Modifications | Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137. Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation with SUMO1 has no effect on transcriptional activity. Acetylated at 2 conserved lysine-rich motifs by CREBBP in vitro. Acetylation does not affect DNA-binding in vitro but is essential to induce erythroid differentiation and for binding chromatin in vivo. Acetylated on Lys-233, Lys-245 Lys-246 by EP300. |
| Function | Transcriptional activator or repressor which serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-ATGATAAG-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS. |
| Protein Name | Erythroid Transcription FactorEryf1Gata-Binding Factor 1Gata-1Gf-1Nf-E1 Dna-Binding Protein |
| Database Links | Reactome: R-HSA-8936459Reactome: R-HSA-8939236Reactome: R-HSA-983231 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Erythroid Transcription Factor antibodyAnti-Eryf1 antibodyAnti-Gata-Binding Factor 1 antibodyAnti-Gata-1 antibodyAnti-Gf-1 antibodyAnti-Nf-E1 Dna-Binding Protein antibodyAnti-GATA1 antibodyAnti-ERYF1 antibodyAnti-GF1 antibody |
Information sourced from Uniprot.org