• STJ70751 (1µg/ml) staining of K562 nuclear cell lysate (A) and negative control Human Hippocampus (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70751 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70751 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70751 Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-GATA1 antibody (Internal) (STJ70751)

SKU:
STJ70751-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-GATA1 (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
IF-Strong expression of the protein seen in the nuclei of HeLa and NIH3T3 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: GATA1
Gene ID: 2623
Uniprot ID: GATA1_HUMAN
Immunogen Region: Internal
Accession Number: NP_002040.1
Immunogen Sequence: DAEAYRHSPVFQ
Post Translational Modifications Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137. Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation with SUMO1 has no effect on transcriptional activity. Acetylated at 2 conserved lysine-rich motifs by CREBBP in vitro. Acetylation does not affect DNA-binding in vitro but is essential to induce erythroid differentiation and for binding chromatin in vivo. Acetylated on Lys-233, Lys-245 Lys-246 by EP300.
Function Transcriptional activator or repressor which serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-ATGATAAG-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS.
Protein Name Erythroid Transcription Factor
Eryf1
Gata-Binding Factor 1
Gata-1
Gf-1
Nf-E1 Dna-Binding Protein
Database Links Reactome: R-HSA-8936459
Reactome: R-HSA-8939236
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Erythroid Transcription Factor antibody
Anti-Eryf1 antibody
Anti-Gata-Binding Factor 1 antibody
Anti-Gata-1 antibody
Anti-Gf-1 antibody
Anti-Nf-E1 Dna-Binding Protein antibody
Anti-GATA1 antibody
Anti-ERYF1 antibody
Anti-GF1 antibody

Information sourced from Uniprot.org

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