Anti-GAPDH antibody (STJ197380)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant Protein of GAPDH
General Information
| Short Description | Rabbit polyclonal anti-Glyceraldehyde-3-phosphate dehydrogenase for use in WB, IHC and IF in Human, Mouse, Rat, Rabbit, Monkey, Sheep, Fish, Chicken, Guinea Pig and Duck samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat/Rabbit/Monkey/Sheep/Fish/Chicken/Guinea Pig/Duck |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:5000IHC 1:200IF 1:50-200 |
| Formulation | Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GAPDH |
| Gene ID | 2597 |
| Uniprot ID | G3P_HUMAN |
| Immunogen | Recombinant Protein of GAPDH |
| Specificity | The antibody detects endogenous GAPDH protein. |
Additional Info
| Function | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. |
| Protein Name | Glyceraldehyde-3-Phosphate DehydrogenaseGapdhPeptidyl-Cysteine S-Nitrosylase Gapdh |
| Database Links | Reactome: R-HSA-70171Reactome: R-HSA-70263 |
| Cellular Localisation | CytoplasmCytosolNucleusPerinuclear RegionMembraneCytoskeletonTranslocates To The Nucleus Following S-Nitrosylation And Interaction With Siah1Which Contains A Nuclear Localization SignalPostnuclear And Perinuclear Regions |
| Alternative Antibody Names | Anti-Glyceraldehyde-3-Phosphate Dehydrogenase antibodyAnti-Gapdh antibodyAnti-Peptidyl-Cysteine S-Nitrosylase Gapdh antibodyAnti-GAPDH antibodyAnti-GAPD antibodyAnti-CDABP0047 antibodyAnti-OK antibodyAnti-SW-cl.12 antibody |
Information sourced from Uniprot.org