• Western blot analysis of extracts of various cell lines, using GALNT2 antibody (STJ28990) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using GALNT2 rabbit polyclonal antibody (STJ28990) at dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using GALNT2 antibody (STJ28990) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using GALNT2 antibody (STJ28990) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using GALNT2 antibody (STJ28990) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-GALNT2 antibody (442-571) (STJ28990)

SKU:
STJ28990

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GALNT2 (442-571) is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
IHC-P 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GALNT2
Gene ID: 2590
Uniprot ID: GALT2_HUMAN
Immunogen Region: 442-571
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 442-571 of human GALNT2 (NP_004472.1).
Immunogen Sequence: HQDIAFGALQQGTNCLDTLG HFADGVVGVYECHNAGGNQE WALTKEKSVKHMDLCLTVVD RAPGSLIKLQGCRENDSRQK WEQIEGNSKLRHVGSNLCLD SRTAKSGGLSVEVCGPALSQ QWKFTLNLQQ
Tissue Specificity Widely expressed.
Function Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b. Probably involved in O-linked glycosylation of the immunoglobulin A1 (IgA1) hinge region. Involved in O-linked glycosylation of APOC-III, ANGPTL3 and PLTP. It participates in the regulation of HDL-C metabolism.
Protein Name Polypeptide N-Acetylgalactosaminyltransferase 2
Polypeptide Galnac Transferase 2
Galnac-T2
Pp-Gantase 2
Protein-Udp Acetylgalactosaminyltransferase 2
Udp-Galnac -Polypeptide N-Acetylgalactosaminyltransferase 2 Cleaved Into - Polypeptide N-Acetylgalactosaminyltransferase 2 Soluble Form
Database Links Reactome: R-HSA-6811436
Reactome: R-HSA-913709
Cellular Localisation Golgi Apparatus
Golgi Stack Membrane
Single-Pass Type Ii Membrane Protein
Secreted
Resides Preferentially In The Trans And Medial Parts Of The Golgi Stack
A Secreted Form Also Exists
Alternative Antibody Names Anti-Polypeptide N-Acetylgalactosaminyltransferase 2 antibody
Anti-Polypeptide Galnac Transferase 2 antibody
Anti-Galnac-T2 antibody
Anti-Pp-Gantase 2 antibody
Anti-Protein-Udp Acetylgalactosaminyltransferase 2 antibody
Anti-Udp-Galnac -Polypeptide N-Acetylgalactosaminyltransferase 2 Cleaved Into - Polypeptide N-Acetylgalactosaminyltransferase 2 Soluble Form antibody
Anti-GALNT2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance