| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Trna (170-329) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FTSJ1 |
| Gene ID: |
24140 |
| Uniprot ID: |
TRM7_HUMAN |
| Immunogen Region: |
170-329 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 170-329 of human FTSJ1 (NP_036412.1). |
| Immunogen Sequence: |
LQVFFSSVLCAKPRSSRNSS IEAFAVCQGYDPPEGFIPDL SKPLLDHSYDPDFNQLDGPT RIIVPFVTCGDLSSYDSDRS YPLDLEGGSEYKYTPPTQPP ISPPYQEACTLKRKGQLAKE IRPQDCPISRVDTFPQPLAA PQCHTLLAPEMEDNEMSCSP |
| Tissue Specificity | Found in fetal brain, lung, liver and kidney. Widely expressed in adult tissue.with high expression in heart and liver, lower expression in skeletal muscle, kidney, and pancreas and also lowly expressed in brain and lung. In the adult brain, expressed in amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus. |
| Function | Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs. Requisite for faithful cytoplasmic translation. Requires THADA for methylation of the nucleotide at position 32 of the anticodon loop of substrate tRNAs. Requires WDR6 for methylation of the nucleotide at position 34 of the anticodon loop of substrate tRNAs. Promotes translation efficiency of the UUU codon. Plays a role in neurogenesis. Required for expression of genes involved in neurogenesis, mitochondrial translation and energy generation, and lipid biosynthesis. Requisite for RNA-mediated gene silencing. May modify position 32 in tRNA(Arg(ACG)), tRNA(Arg(CCG)), tRNA(Arg(UCG)), tRNA(Cys(GCA)), tRNA(Cys(ACA)), tRNA(Gln(CUG)), tRNA(Gln(UUG)), tRNA(Gly(CCC)), tRNA(Leu(CAG))/tRNA(Leu(CAA)), tRNA(Leu(A/IAG)), tRNA(Leu(UAG)), tRNA(Phe(GAA)), tRNA(Pro(AGG))/tRNA(Pro(CGG))/tRNA(Pro(UGG)) and tRNA(Trp(CCA)), and position 34 in tRNA(Phe(GAA)), tRNA(Leu(CAA)), tRNA(Sec(UCA)), and tRNA(Trp(CCA)). |
| Protein Name | TrnaCytidine(32/Guanosine(34-2'-O-Methyltransferase2'-O-Ribose Rna Methyltransferase Trm7 HomologProtein Ftsj Homolog 1 |
| Database Links | Reactome: R-HSA-6782315 |
| Cellular Localisation | CytoplasmNucleusPredominantly Cytoplasmic |
| Alternative Antibody Names | Anti-Trna antibodyAnti-Cytidine(32/Guanosine(34-2'-O-Methyltransferase antibodyAnti-2'-O-Ribose Rna Methyltransferase Trm7 Homolog antibodyAnti-Protein Ftsj Homolog 1 antibodyAnti-FTSJ1 antibodyAnti-JM23 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and CRYZ knockout (KO) HeLa cells, using [KO Validated] CRYZ Rabbit polyclonal antibody (STJ11100873) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89807/358274/STJ11100873_1__03594.1713122620.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CRYZ knockout (KO) HeLa cells, using [KO Validated] CRYZ Rabbit polyclonal antibody (STJ11100873) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
