Western blot analysis of various lysates, using FTSJ1 Rabbit pAb (STJ11105734) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-FTSJ1 antibody (170-329) (STJ11105734)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11105734
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General Information

Short DescriptionRabbit polyclonal Trna (170-329) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolFTSJ1
Gene ID24140
Uniprot IDTRM7_HUMAN
Immunogen Region170-329
Immunogen SequenceLQVFFSSVLCAKPRSSRNSS IEAFAVCQGYDPPEGFIPDL SKPLLDHSYDPDFNQLDGPT RIIVPFVTCGDLSSYDSDRS YPLDLEGGSEYKYTPPTQPP ISPPYQEACTLKRKGQLAKE IRPQDCPISRVDTFPQPLAA PQCHTLLAPEMEDNEMSCSP
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 170-329 of human FTSJ1 (NP_036412.1).

Additional Info

Tissue Specificity Found in fetal brain, lung, liver and kidney. Widely expressed in adult tissue.with high expression in heart and liver, lower expression in skeletal muscle, kidney, and pancreas and also lowly expressed in brain and lung. In the adult brain, expressed in amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus.
Function Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs. Requisite for faithful cytoplasmic translation. Requires THADA for methylation of the nucleotide at position 32 of the anticodon loop of substrate tRNAs. Requires WDR6 for methylation of the nucleotide at position 34 of the anticodon loop of substrate tRNAs. Promotes translation efficiency of the UUU codon. Plays a role in neurogenesis. Required for expression of genes involved in neurogenesis, mitochondrial translation and energy generation, and lipid biosynthesis. Requisite for RNA-mediated gene silencing. May modify position 32 in tRNA(Arg(ACG)), tRNA(Arg(CCG)), tRNA(Arg(UCG)), tRNA(Cys(GCA)), tRNA(Cys(ACA)), tRNA(Gln(CUG)), tRNA(Gln(UUG)), tRNA(Gly(CCC)), tRNA(Leu(CAG))/tRNA(Leu(CAA)), tRNA(Leu(A/IAG)), tRNA(Leu(UAG)), tRNA(Phe(GAA)), tRNA(Pro(AGG))/tRNA(Pro(CGG))/tRNA(Pro(UGG)) and tRNA(Trp(CCA)), and position 34 in tRNA(Phe(GAA)), tRNA(Leu(CAA)), tRNA(Sec(UCA)), and tRNA(Trp(CCA)).
Protein Name Trna
Cytidine(32/Guanosine(34-2'-O-Methyltransferase
2'-O-Ribose Rna Methyltransferase Trm7 Homolog
Protein Ftsj Homolog 1
Database Links Reactome: R-HSA-6782315
Cellular Localisation Cytoplasm
Nucleus
Predominantly Cytoplasmic
Alternative Antibody Names Anti-Trna antibody
Anti-Cytidine(32/Guanosine(34-2'-O-Methyltransferase antibody
Anti-2'-O-Ribose Rna Methyltransferase Trm7 Homolog antibody
Anti-Protein Ftsj Homolog 1 antibody
Anti-FTSJ1 antibody
Anti-JM23 antibody

Information sourced from Uniprot.org

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