• Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Fascin/Fascin/FSCN1 Rabbit polyclonal antibody (STJ23712) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using [KO Validated] Fascin/Fascin/FSCN1 Rabbit polyclonal antibody (STJ23712) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using [KO Validated] Fascin/FSCN1 Rabbit polyclonal antibody (STJ23712) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts from normal (control) and Fascin/Fascin/FSCN1 knockout (KO) HeLa cells, using Fascin/Fascin/FSCN1 antibody (STJ23712) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of extracts of various cell lines, using Fascin/Fascin/FSCN1 antibody (STJ23712) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-FSCN1 antibody (394-493) (STJ23712)

SKU:
STJ23712

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FSCN1 (394-493) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FSCN1
Gene ID: 6624
Uniprot ID: FSCN1_HUMAN
Immunogen Region: 394-493
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 394-493 of human Fascin/Fascin/FSCN1 (NP_003079.1).
Immunogen Sequence: FIGCRKVTGTLDANRSSYDV FQLEFNDGAYNIKDSTGKYW TVGSDSAVTSSGDTPVDFFF EFCDYNKVAIKVGGRYLKGD HAGVLKASAETVDPASLWEY
Tissue Specificity Ubiquitous.
Post Translational Modifications Phosphorylation at Ser-39 inhibits actin-binding. Phosphorylation is required for the reorganization of the actin cytoskeleton in response to NGF.
Function Actin-binding protein that contains 2 major actin binding sites. Organizes filamentous actin into parallel bundles. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF.
Protein Name Fascin
55 Kda Actin-Bundling Protein
Singed-Like Protein
P55
Database Links Reactome: R-HSA-6785807
Cellular Localisation Cytoplasm
Cytosol
Cell Cortex
Cytoskeleton
Stress Fiber
Cell Projection
Filopodium
Invadopodium
Microvillus
Cell Junction
Colocalized With Rufy3 And F-Actin At Filipodia Of The Axonal Growth Cone
Colocalized With Dbn1 And F-Actin At The Transitional Domain Of The Axonal Growth Cone
Alternative Antibody Names Anti-Fascin antibody
Anti-55 Kda Actin-Bundling Protein antibody
Anti-Singed-Like Protein antibody
Anti-P55 antibody
Anti-FSCN1 antibody
Anti-FAN1 antibody
Anti-HSN antibody
Anti-SNL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance