• Western blot analysis of the lysates from HUVECcells using FZD7 antibody.
  • Western blot analysis of lysates from HT-29 cells, using FZD7 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of HELA cells using Frizzled-7 Polyclonal Antibody diluted at 1:2000
  • Immunofluorescence analysis of MCF7 cells, using FZD7 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using Frizzled-7 Polyclonal Antibody diluted at 1:2000

Anti-Frizzled-7 antibody (STJ93151)

SKU:
STJ93151

Current Stock:
Host: Rabbit
Applications: WB, IF, ELISA
Reactivity: Human, Mouse
Short Description: Rabbit polyclonal anti-Frizzled-7 antibody is suitable for use in Western Blot, Immunofluorescence and ELISA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:20000
Specificity: Frizzled-7 polyclonal antibody detects endogenous levels of Frizzled-7 protein.
Uniprot ID: FZD7_HUMAN
Gene ID: 8324
Gene Symbol: FZD7
Immunogen: Synthesized peptide derived from human Frizzled-7
Immunogen Region: 20-100 aa, N-terminal
Post Translational Modifications Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
Function Receptor for Wnt proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Activation by WNT8 induces expression of beta-catenin target genes. Following ligand activation, binds to CCDC88C/DAPLE which displaces DVL1 from FZD7 and leads to inhibition of canonical Wnt signaling, activation of G-proteins by CCDC88C and triggering of non-canonical Wnt responses. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. (Microbial infection) Acts as a receptor for C.difficile toxin TcdB in the colonic epithelium.
Protein Name Frizzled-7
Fz-7
Hfz7
Fze3
Database Links Reactome: R-HSA-373080
Reactome: R-HSA-4086400
Reactome: R-HSA-4608870
Reactome: R-HSA-9673324
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Endosome Membrane
Associated To The Plasma Membrane In The Presence Of Fzd7 And Phosphatidylinositol 4
5-Bisphosphate (Pip2)
Localized In Recycling Endosomes In Other Conditions
Alternative Antibody Names Anti-Frizzled-7 antibody
Anti-Fz-7 antibody
Anti-Hfz7 antibody
Anti-Fze3 antibody
Anti-FZD7 antibody

Information sourced from Uniprot.org

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