||WB, IF, ELISA
||Rabbit polyclonal anti-Frizzled-7 antibody is suitable for use in Western Blot, Immunofluorescence and ELISA.
||FOR RESEARCH USE ONLY (RUO).
||Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
||Store at-20°C, and avoid repeat freeze-thaw cycles.
||WB 1:500-1:2000IF 1:200-1:1000ELISA 1:20000
||Frizzled-7 polyclonal antibody detects endogenous levels of Frizzled-7 protein.
||Synthesized peptide derived from human Frizzled-7
||20-100 aa, N-terminal
| Post Translational Modifications || Ubiquitinated by ZNRF3, leading to its degradation by the proteasome. |
| Function || Receptor for Wnt proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Activation by WNT8 induces expression of beta-catenin target genes. Following ligand activation, binds to CCDC88C/DAPLE which displaces DVL1 from FZD7 and leads to inhibition of canonical Wnt signaling, activation of G-proteins by CCDC88C and triggering of non-canonical Wnt responses. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. (Microbial infection) Acts as a receptor for C.difficile toxin TcdB in the colonic epithelium. |
| Protein Name || Frizzled-7Fz-7Hfz7Fze3 |
| Database Links || Reactome: R-HSA-373080Reactome: R-HSA-4086400Reactome: R-HSA-4608870Reactome: R-HSA-9673324 |
| Cellular Localisation || Cell MembraneMulti-Pass Membrane ProteinEndosome MembraneAssociated To The Plasma Membrane In The Presence Of Fzd7 And Phosphatidylinositol 45-Bisphosphate (Pip2)Localized In Recycling Endosomes In Other Conditions |
| Alternative Antibody Names || Anti-Frizzled-7 antibodyAnti-Fz-7 antibodyAnti-Hfz7 antibodyAnti-Fze3 antibodyAnti-FZD7 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance