• Immunofluorescence analysis of NIH/3T3 cells using FREM2 Rabbit polyclonal antibody (STJ118425) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using FREM2 Rabbit polyclonal antibody (STJ118425) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 150s.

Anti-FREM2 antibody (47-300) (STJ118425)

SKU:
STJ118425

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FREM2 (47-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FREM2
Gene ID: 341640
Uniprot ID: FREM2_HUMAN
Immunogen Region: 47-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 47-300 of human FREM2 (NP_997244.4).
Immunogen Sequence: QPAAFGRALLSPGLAGAAGV PAEEAIVLANRGLRVPFGRE VWLDPLHDLVLQVQPGDRCA VSVLDNDALAQRPGRLSPKR FPCDFGPGEVRYSHLGARSP SRDRVRLQLRYDAPGGAVVL PLVLEVEVVFTQLEVVTRNL PLVVEELLGTSNALDARSLE FAFQPETEECRVGILSGLGA LPRYGELLHYPQVPGGAREG GAPETLLMDCKAFQELGVRY RHTAASRSPNRDWIPMVVE
Function Extracellular matrix protein required for maintenance of the integrity of the skin epithelium and for maintenance of renal epithelia. Required for epidermal adhesion. Involved in the development of eyelids and the anterior segment of the eyeballs.
Protein Name Fras1-Related Extracellular Matrix Protein 2
Ecm3 Homolog
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Fras1-Related Extracellular Matrix Protein 2 antibody
Anti-Ecm3 Homolog antibody
Anti-FREM2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance