• Western blot analysis of extracts of HeLa cells, using FOSL1 antibody (STJ27325) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of C6 cells, using FOSL1 antibody (STJ27325) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-FOSL1 antibody (1-100) (STJ27325)

SKU:
STJ27325

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FOSL1 (1-100) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FOSL1
Gene ID: 8061
Uniprot ID: FOSL1_HUMAN
Immunogen Region: 1-100
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human FOSL1 (NP_005429.1).
Immunogen Sequence: MFRDFGEPGPSSGNGGGYGG PAQPPAAAQAAQQKFHLVPS INTMSGSQELQWMVQPHFLG PSSYPRPLTYPQYSPPQPRP GVIRALGPPPGVRRRPCEQI
Protein Name Fos-Related Antigen 1
Fra-1
Database Links Reactome: R-HSA-9031628
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Fos-Related Antigen 1 antibody
Anti-Fra-1 antibody
Anti-FOSL1 antibody
Anti-FRA1 antibody

Information sourced from Uniprot.org

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