• Immunohistochemistry analysis of paraffin-embedded human liver cancer using FLT3 antibody (STJ114311) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat pancreas using FLT3 antibody (STJ114311) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using FLT3 antibody (STJ114311) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-FLT3 antibody (701-800) (STJ114311)

SKU:
STJ114311

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FLT3 (701-800) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FLT3
Gene ID: 2322
Uniprot ID: FLT3_HUMAN
Immunogen Region: 701-800
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 701-800 of human FLT3 (NP_004110.2).
Immunogen Sequence: NYLRSKREKFHRTWTEIFKE HNFSFYPTFQSHPNSSMPGS REVQIHPDSDQISGLHGNSF HSEDEIEYENQKRLEEEEDL NVLTFEDLLCFAYQVAKGME
Tissue Specificity Detected in bone marrow, in hematopoietic stem cells, in myeloid progenitor cells and in granulocyte/macrophage progenitor cells (at protein level). Detected in bone marrow, liver, thymus, spleen and lymph node, and at low levels in kidney and pancreas. Highly expressed in T-cell leukemia.
Post Translational Modifications N-glycosylated, contains complex N-glycans with sialic acid. Autophosphorylated on several tyrosine residues in response to FLT3LG binding. FLT3LG binding also increases phosphorylation of mutant kinases that are constitutively activated. Dephosphorylated by PTPRJ/DEP-1, PTPN1, PTPN6/SHP-1, and to a lesser degree by PTPN12. Dephosphorylation is important for export from the endoplasmic reticulum and location at the cell membrane. Rapidly ubiquitinated by UBE2L6 and the E3 ubiquitin-protein ligase SIAH1 after autophosphorylation, leading to its proteasomal degradation.
Function Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.
Protein Name Receptor-Type Tyrosine-Protein Kinase Flt3
Fl Cytokine Receptor
Fetal Liver Kinase-2
Flk-2
Fms-Like Tyrosine Kinase 3
Flt-3
Stem Cell Tyrosine Kinase 1
Stk-1
Cd Antigen Cd135
Database Links Reactome: R-HSA-109704
Reactome: R-HSA-1257604
Reactome: R-HSA-2219530
Reactome: R-HSA-5673001
Reactome: R-HSA-6811558
Reactome: R-HSA-9607240
Reactome: R-HSA-9645135
Reactome: R-HSA-9702509
Reactome: R-HSA-9702518
Reactome: R-HSA-9702569
Reactome: R-HSA-9702577
Reactome: R-HSA-9702581
Reactome: R-HSA-9702590
Reactome: R-HSA-9702596
Reactome: R-HSA-9702600
Reactome: R-HSA-9702605
Reactome: R-HSA-9702614
Reactome: R-HSA-9702620
Reactome: R-HSA-9702624
Reactome: R-HSA-9702632
Reactome: R-HSA-9702636
Reactome: R-HSA-9702998
Reactome: R-HSA-9703009
Reactome: R-HSA-9703648
Reactome: R-HSA-9706369
Reactome: R-HSA-9706374
Reactome: R-HSA-9706377
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Endoplasmic Reticulum Lumen
Constitutively Activated Mutant Forms With Internal Tandem Duplications Are Less Efficiently Transported To The Cell Surface And A Significant Proportion Is Retained In An Immature Form In The Endoplasmic Reticulum Lumen
The Activated Kinase Is Rapidly Targeted For Degradation
Alternative Antibody Names Anti-Receptor-Type Tyrosine-Protein Kinase Flt3 antibody
Anti-Fl Cytokine Receptor antibody
Anti-Fetal Liver Kinase-2 antibody
Anti-Flk-2 antibody
Anti-Fms-Like Tyrosine Kinase 3 antibody
Anti-Flt-3 antibody
Anti-Stem Cell Tyrosine Kinase 1 antibody
Anti-Stk-1 antibody
Anti-Cd Antigen Cd135 antibody
Anti-FLT3 antibody
Anti-CD135 antibody
Anti-FLK2 antibody
Anti-STK1 antibody

Information sourced from Uniprot.org

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