• Western blot analysis using Flt-1 monoclonal antibody against extracellular domain of human Flt-1.

Anti-FLT1 antibody [3D10] (STJ98080)

SKU:
STJ98080

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Vascular endothelial growth factor receptor 1 is suitable for use in Western Blot and ELISA research applications.
Clonality: Monoclonal
Clone ID: 3D10
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FLT1
Gene ID: 2321
Uniprot ID: VGFR1_HUMAN
Specificity: Flt-1 Monoclonal Antibody detects endogenous levels of Flt-1 protein.
Immunogen: Purified recombinant extracellular fragment of human Flt-1 fused with hIgGFc tag expressed in HEK293 cells.
Post Translational Modifications N-glycosylated. Ubiquitinated after VEGFA-mediated autophosphorylation, leading to proteolytic degradation. Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-1169 is important for interaction with PLCG. Phosphorylation at Tyr-1213 is important for interaction with PIK3R1, PTPN11, GRB2, and PLCG. Phosphorylation at Tyr-1333 is important for endocytosis and for interaction with CBL, NCK1 and CRK. Is probably dephosphorylated by PTPRB.
Function Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Acts as a positive regulator of postnatal retinal hyaloid vessel regression. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro). Has very high affinity for VEGFA and relatively low protein kinase activity.may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC and YES1, and may also phosphorylate CBL. Promotes phosphorylation of AKT1 at 'Ser-473'. Promotes phosphorylation of PTK2/FAK1. Isoform 1: Phosphorylates PLCG. Isoform 2: May function as decoy receptor for VEGFA. Isoform 3: May function as decoy receptor for VEGFA. Isoform 4: May function as decoy receptor for VEGFA. Isoform 7: Has a truncated kinase domain.it increases phosphorylation of SRC at 'Tyr-418' by unknown means and promotes tumor cell invasion.
Protein Name Vascular Endothelial Growth Factor Receptor 1
Vegfr-1
Fms-Like Tyrosine Kinase 1
Flt-1
Tyrosine-Protein Kinase Frt
Tyrosine-Protein Kinase Receptor Flt
Flt
Vascular Permeability Factor Receptor
Database Links Reactome: R-HSA-194306
Reactome: R-HSA-195399
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Endosome
Autophosphorylation Promotes Ubiquitination And Endocytosis
Isoform 2: Secreted
Isoform 3: Secreted
Isoform 4: Secreted
Isoform 5: Cytoplasm
Isoform 6: Cytoplasm
Isoform 7: Cytoplasm
Alternative Antibody Names Anti-Vascular Endothelial Growth Factor Receptor 1 antibody
Anti-Vegfr-1 antibody
Anti-Fms-Like Tyrosine Kinase 1 antibody
Anti-Flt-1 antibody
Anti-Tyrosine-Protein Kinase Frt antibody
Anti-Tyrosine-Protein Kinase Receptor Flt antibody
Anti-Flt antibody
Anti-Vascular Permeability Factor Receptor antibody
Anti-FLT1 antibody
Anti-FLT antibody
Anti-FRT antibody
Anti-VEGFR1 antibody

Information sourced from Uniprot.org

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