• Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded human tonsil tissue using Flotillin 1 Rabbit monoclonal antibody (STJ11101835) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded human thyroid cancer tissue using Flotillin 1 Rabbit monoclonal antibody (STJ11101835) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded human liver cancer tissue using Flotillin 1 Rabbit monoclonal antibody (STJ11101835) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates, using Flotillin 1 Rabbit monoclonal antibody (STJ11101835) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of various lysates, using Flotillin 1 Rabbit monoclonal antibody (STJ11101835) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-FLOT1 antibody (328-427) [S5MR] (STJ11101835)

SKU:
STJ11101835

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Flotillin 1 (328-427) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000 IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FLOT1
Gene ID: 10211
Uniprot ID: FLOT1_HUMAN
Immunogen Region: 328-427
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 328-427 of human Flotillin 1 (O75955).
Immunogen Sequence: GARARAEAEQMAKKAEAFQL YQEAAQLDMLLEKLPQVAEE ISGPLTSANKITLVSSGSGT MGAAKVTGEVLDILTRLPES VERLTGVSISQVNHKPLRTA
Function May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Protein Name Flotillin-1
Database Links Reactome: R-HSA-5213460
Reactome: R-HSA-5675482
Reactome: R-HSA-8849932
Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Endosome
Membrane
Caveola
Melanosome
Membrane Raft
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Membrane-Associated Protein Of Caveola
Alternative Antibody Names Anti-Flotillin-1 antibody
Anti-FLOT1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance