• Immunohistochemistry analysis of FLII in paraffin-embedded mouse heart using FLII Rabbit polyclonal antibody (STJ117468) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of FLII in paraffin-embedded human colon carcinoma using FLII Rabbit polyclonal antibody (STJ117468) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of FLII in paraffin-embedded rat heart using FLII Rabbit polyclonal antibody (STJ117468) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using FLII Rabbit polyclonal antibody (STJ117468) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5S.

Anti-FLII antibody (400-660) (STJ117468)

SKU:
STJ117468

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FLII (400-660) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FLII
Gene ID: 2314
Uniprot ID: FLII_HUMAN
Immunogen Region: 400-660
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human FLII (NP_002009.1).
Immunogen Sequence: LRLAGASPATVAAAAAAGSG PKDPMARKMRLRRRKDSAQD DQAKQVLKGMSDVAQEKNKK QEESADARAPSGKVRRWDQG LEKPRLDYSEFFTEDVGQLP GLTIWQIENFVPVLVEEAFH GKFYEADCYIVLKTFLDDSG SLNWEIYYWIGGEATLDKKA CSAIHAVNLRNYLGAECRTV REEMGDESEEFLQVFDNDIS YIEGGTASGFYTVEDTHYVT RMYRVYGKKNIKLEPVPLK
Tissue Specificity Strongest expression in skeletal muscle with high expression also in the heart and lung.
Function May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development. May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation.
Protein Name Protein Flightless-1 Homolog
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Cell Junction
Focal Adhesion
Colocalizes To Actin-Rich Structures In Blastocysts And
Together With Hras
Rhoa And Cdc42
In Migrating Fibroblasts
Localizes To Centrosomes
Alternative Antibody Names Anti-Protein Flightless-1 Homolog antibody
Anti-FLII antibody
Anti-FLIL antibody

Information sourced from Uniprot.org

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