Anti-FLG antibody (1-92) (STJ11101786)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal FLG (1-92) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | FLG |
| Gene ID | 2312 |
| Uniprot ID | FILA_HUMAN |
| Immunogen Region | 1-92 |
| Immunogen Sequence | MSTLLENIFAIINLFKQYSK KDKNTDTLSKKELKELLEKE FRQILKNPDDPDMVDVFMDH LDIDHNKKIDFTEFLLMVFK LAQAYYESTRKE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human FLG (NP_002007.1). |
Additional Info
| Tissue Specificity | Expressed in skin, thymus, stomach, tonsils, testis, placenta, kidney, pancreas, mammary gland, bladder, thyroid, salivary gland and trachea, but not detected in heart, brain, liver, lung, bone marrow, small intestine, spleen, prostate, colon, or adrenal gland. In the skin, mainly expressed in stratum granulosum of the epidermis. |
| Post Translational Modifications | Filaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate. Undergoes deimination of some arginine residues (citrullination). |
| Function | Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis. |
| Protein Name | Filaggrin |
| Database Links | Reactome: R-HSA-6809371Reactome: R-HSA-9725554 |
| Cellular Localisation | Cytoplasmic GranuleIn The Stratum Granulosum Of The EpidermisLocalized Within Keratohyalin GranulesIn Granular Keratinocytes And In Lower CorneocytesColocalizes With Calpain-1/Capn1 |
| Alternative Antibody Names | Anti-Filaggrin antibodyAnti-FLG antibody |
Information sourced from Uniprot.org