Biotinylated STJ71006 (0. 3ยตg/ml) staining of U937 lysate (35ยตg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-FLAP/ALOX5AP, Biotinylated antibody (Internal) {Biotin} (STJ73174)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationBiotin
IsotypeIgG
STJ73174-100
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General Information

Short DescriptionGoat polyclonal antibody anti-FLAP/ALOX5AP, Biotinylated (Internal) is suitable for use in ELISA and Western Blot research applications.
ApplicationsPep-ELISA/WB
HostGoat
ReactivityHuman/Dog
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationBiotin
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:128000.
WB: Approx 16kDa band observed in lysates of cell line U937 (calculated MW of 18.2kDa according to NP_001620.2). See non-biotinylated parental product's datasheet for further QC data. Rec
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolALOX5AP
Gene ID241
Uniprot IDAL5AP_HUMAN
Accession NumberNP_001620.2; NP_001191335.1
Immunogen RegionInternal
Immunogen SequenceHKVEHESRTQNGR.
SpecificityThis antibody is expected to recognize both reported isoforms (NP_001620.2; NP_001191335.1).

Additional Info

Protein Name Arachidonate 5-Lipoxygenase-Activating Protein
Flap
Mk-886-Binding Protein
Database Links Reactome: R-HSA-2142688
Reactome: R-HSA-2142691
Reactome: R-HSA-2142700
Cellular Localisation Nucleus Membrane
Multi-Pass Membrane Protein
Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Arachidonate 5-Lipoxygenase-Activating Protein antibody
Anti-Flap antibody
Anti-Mk-886-Binding Protein antibody
Anti-ALOX5AP antibody
Anti-FLAP antibody

Information sourced from Uniprot.org

Citations

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