• STJ70560 (6µg/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
  • STJ70560 Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
  • STJ70560 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing strong endplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70560 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-FLAP/ALOX5AP antibody (C-Term) (STJ70560)

SKU:
STJ70560-100

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Host: Goat
Applications: Pep-ELISA/IHC/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-FLAP/ALOX5AP (C-Term) is suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-6µg/ml
IF-Strong expression of the protein seen in the endplasmic reticulum of MCF7 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ALOX5AP
Gene ID: 241
Uniprot ID: AL5AP_HUMAN
Immunogen Region: C-Term
Accession Number: NP_001620.2; NP_001191335.1
Immunogen Sequence: KTISTTISPLLLIP
Protein Name Arachidonate 5-Lipoxygenase-Activating Protein
Flap
Mk-886-Binding Protein
Database Links Reactome: R-HSA-2142688
Reactome: R-HSA-2142691
Reactome: R-HSA-2142700
Cellular Localisation Nucleus Membrane
Multi-Pass Membrane Protein
Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Arachidonate 5-Lipoxygenase-Activating Protein antibody
Anti-Flap antibody
Anti-Mk-886-Binding Protein antibody
Anti-ALOX5AP antibody
Anti-FLAP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance