Anti-Flag-Tag antibody [12M11] (STJA0006221)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenSynthetic Peptide of Flag-Tag
General Information
| Short Description | Mouse monoclonal anti-Flag-Tag-Tag for use in WB, ELISA, IP, IF and IP in Species independent samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA/IP/IF/IP |
| Host | Mouse |
| Reactivity | Species independent |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 12M11 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | Protein A purified. |
| Dilution Range | WB 1:10000-50000ELISA 1:20000-50000IP 1:50-1:200IF 1:1000-3000 |
| Formulation | Liquid in PBS pH7.4, containing 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Immunogen | Synthetic Peptide of Flag-Tag |
| Specificity | This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library i |
Additional Info
| Background | The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. |
Information sourced from Uniprot.org