FLAG-Tag Antibodies
Detection of FLAG-tagged fusion proteins carrying the eight-residue DYKDDDDK peptideThe FLAG octapeptide (DYKDDDDK) is a short, hydrophilic marker sequence engineered onto the N-terminus of recombinant proteins to aid their detection and purification, using an antibody raised against the first four residues of the tag.1 Because the peptide is small and unrelated to the biology of most fusion partners, tagged proteins generally retain their biological activity.
The tag can be added to either the N- or C-terminus of a protein, or used in tandem repeats, and its mild elution conditions make it suitable for isolating whole multi-subunit protein complexes rather than just the tagged protein alone. Our range covers rabbit polyclonal and mouse monoclonal formats.
Anti-FLAG-Tag Antibodies
Polyclonal and monoclonal antibodies against the FLAG tag, covering Western Blot, ELISA, immunoprecipitation and immunofluorescence.
Anti-FLAG-Tag antibody
Rabbit polyclonal antibody raised against the DYKDDDDK peptide, detecting FLAG-tagged recombinant proteins or FLAG-tagged proteins overexpressed in cells.
View product →
Anti-Flag-Tag antibody [2C5]
Mouse monoclonal antibody suitable for use in Western Blot, immunoprecipitation, immunofluorescence and immunocytochemistry.
View product →Looking for a Different Tag?
We supply antibodies against FLAG-Tag, His-Tag, DDDDK-Tag, HA-Tag, Avi-Tag, Myc-Tag, GST-Tag, V5-Tag, T7-Tag, Strep-Tag, S-Tag, GFP-Tag and mCherry-Tag fusion proteins.
References
1. Hopp TP, Prickett KS, Price VL, Libby RT, March CJ, Cerretti DP, Urdal DL, Conlon PJ. A short polypeptide marker sequence useful for recombinant protein identification and purification. Bio/Technology. 1988;6:1204-1210. Link
For Research Use Only. Not for use in diagnostic or therapeutic procedures.