| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-Fibronectin (1-2477) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S9MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:2000-1:6000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FN1 |
| Gene ID: |
2335 |
| Uniprot ID: |
FINC_HUMAN |
| Immunogen Region: |
1-2477 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-2477 of human FN1 (P02751). |
| Immunogen Sequence: |
MLRGPGPGLLLLAVQCLGTA VPSTGASKSKRQAQQMVQPQ SPVAVSQSKPGCYDNGKHYQ INQQWERTYLGNALVCTCYG GSRGFNCESKPEAEETCFDK YTGNTYRVGDTYERPKDSMI WDCTCIGAGRGRISCTIANR CHEGGQSYKIGDTWRRPHET GGYMLECVCLGNGKGEWTCK PIAEKCFDHAAGTSYVVGET WEKPYQGWMMVDCTCLGEGS GRITCTSRNRCNDQDTRTS |
| Tissue Specificity | Expressed in the inner limiting membrane and around blood vessels in the retina (at protein level). Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. |
| Post Translational Modifications | Sulfated. It is not known whether both or only one of Thr-2155 and Thr-2156 are/is glycosylated. Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers). Phosphorylated by FAM20C in the extracellular medium. Proteolytic processing produces the C-terminal NC1 peptide, anastellin. Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation. Serotonylated on Gln residues by TGM2 in response to hypoxia. |
| Function | Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation. Anastellin: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling. |
| Protein Name | FibronectinFnCold-Insoluble GlobulinCig Cleaved Into - Anastellin - Ugl-Y1 - Ugl-Y2 - Ugl-Y3 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1474228Reactome: R-HSA-1474244Reactome: R-HSA-1566977Reactome: R-HSA-202733Reactome: R-HSA-2129379Reactome: R-HSA-216083Reactome: R-HSA-3000170Reactome: R-HSA-3000171Reactome: R-HSA-3000178Reactome: R-HSA-354192Reactome: R-HSA-354194Reactome: R-HSA-372708Reactome: R-HSA-381426Reactome: R-HSA-5674135Reactome: R-HSA-6785807Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-8874081Reactome: R-HSA-8957275Reactome: R-HSA-9634597Reactome: R-HSA-9649948Reactome: R-HSA-9656223Reactome: R-HSA-9700645Reactome: R-HSA-9725370Reactome: R-HSA-9725371 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Antibody Names | Anti-Fibronectin antibodyAnti-Fn antibodyAnti-Cold-Insoluble Globulin antibodyAnti-Cig Cleaved Into - Anastellin - Ugl-Y1 - Ugl-Y2 - Ugl-Y3 antibodyAnti-FN1 antibodyAnti-FN antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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