Anti-Fibrillarin antibody (C-Term) (STJ13100128)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from the c-terminal region of human Fibrillarin conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat, mouse and xenopus.
General Information
| Short Description | Rabbit polyclonal anti-Fibrillarin (C-Term) for use in IHC and WB in Human, Rat, Mouse and Xenopus samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse/Xenopus |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1:1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | FBL |
| Gene ID | 2091 |
| Uniprot ID | FBRL_HUMAN |
| Immunogen | A synthetic peptide from the c-terminal region of human Fibrillarin conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat, mouse and xenopus. |
| Immunogen Region | C-Term |
| Specificity | Specific for Fibrillarin. |
Additional Info
| Function | S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes. U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. |
| Protein Name | Rrna 2'-O-Methyltransferase Fibrillarin34 Kda Nucleolar Scleroderma AntigenHistone-Glutamine MethyltransferaseU6 Snrna 2'-O-Methyltransferase Fibrillarin |
| Database Links | Reactome: R-HSA-6790901Reactome: R-HSA-6791226 |
| Cellular Localisation | NucleusNucleolusNucleoplasmFibrillar Region Of The Nucleolus |
| Alternative Antibody Names | Anti-Rrna 2'-O-Methyltransferase Fibrillarin antibodyAnti-34 Kda Nucleolar Scleroderma Antigen antibodyAnti-Histone-Glutamine Methyltransferase antibodyAnti-U6 Snrna 2'-O-Methyltransferase Fibrillarin antibodyAnti-FBL antibodyAnti-FIB1 antibodyAnti-FLRN antibody |
Information sourced from Uniprot.org