• Western blot analysis of extracts of various cell lines, using FHL3 antibody (STJ111377) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of HeLa cells using FHL3 Rabbit polyclonal antibody (STJ111377) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using FHL3 rabbit polyclonal antibody (STJ111377) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using FHL3 antibody (STJ111377) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-FHL3 antibody (1-280) (STJ111377)

SKU:
STJ111377

£169.50 - £449.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FHL3 (1-280) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FHL3
Gene ID: 2275
Uniprot ID: FHL3_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human FHL3 (NP_004459.2).
Immunogen Sequence: MSESFDCAKCNESLYGRKYI QTDSGPYCVPCYDNTFANTC AECQQLIGHDSRELFYEDRH FHEGCFRCCRCQRSLADEPF TCQDSELLCNDCYCSAFSSQ CSACGETVMPGSRKLEYGGQ TWHEHCFLCSGCEQPLGSRS FVPDKGAHYCVPCYENKFAP RCARCSKTLTQGGVTYRDQP WHRECLVCTGCQTPLAGQQF TSRDEDPYCVACFGELFAPK CSSCKRPIVGLGGGKYVSF
Tissue Specificity Expressed only in skeletal muscle.
Function Recruited by SOX15 to FOXK1 promoters where it acts as a transcriptional coactivator of FOXK1.
Protein Name Four And A Half Lim Domains Protein 3
Fhl-3
Skeletal Muscle Lim-Protein 2
Slim-2
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Four And A Half Lim Domains Protein 3 antibody
Anti-Fhl-3 antibody
Anti-Skeletal Muscle Lim-Protein 2 antibody
Anti-Slim-2 antibody
Anti-FHL3 antibody
Anti-SLIM2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance