• Western blot analysis of extracts of various cell lines, using FHIT rabbit monoclonal antibody (STJ11102418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of various lysates using FHIT Rabbit monoclonal antibody (STJ11102418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-FHIT antibody (1-100) [S8MR] (STJ11102418)

SKU:
STJ11102418

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-FHIT (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FHIT
Gene ID: 2272
Uniprot ID: FHIT_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FHIT (P49789).
Immunogen Sequence: MSFRFGQHLIKPSVVFLKTE LSFALVNRKPVVPGHVLVCP LRPVERFHDLRPDEVADLFQ TTQRVGTVVEKHFHGTSLTF SMQDGPEAGQTVKHVHVHVL
Tissue Specificity Low levels expressed in all tissues tested. Phospho-FHIT observed in liver and kidney, but not in brain and lung. Phospho-FHIT undetected in all tested human tumor cell lines.
Post Translational Modifications Phosphorylation at Tyr-114 by SRC is required for induction of apoptosis.
Function Possesses dinucleoside triphosphate hydrolase activity. Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Exhibits adenylylsulfatase activity, hydrolyzing adenosine 5'-phosphosulfate to yield AMP and sulfate. Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2. Exhibits adenylylsulfate-ammonia adenylyltransferase, catalyzing the ammonolysis of adenosine 5'-phosphosulfate resulting in the formation of adenosine 5'-phosphoramidate. Also catalyzes the ammonolysis of adenosine 5-phosphorofluoridate and diadenosine triphosphate. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low-affinity Ca(2+) transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor.
Protein Name Bis(5'-Adenosyl-Triphosphatase
Ap3a Hydrolase
Ap3aase
Adenosine 5'-Monophosphoramidase Fhit
Adenylylsulfatase
Adenylylsulfate-Ammonia Adenylyltransferase
Diadenosine 5' -5'''-P1 -P3-Triphosphate Hydrolase
Dinucleosidetriphosphatase
Fragile Histidine Triad Protein
Cellular Localisation Cytoplasm
Mitochondrion
Nucleus
Alternative Antibody Names Anti-Bis(5'-Adenosyl-Triphosphatase antibody
Anti-Ap3a Hydrolase antibody
Anti-Ap3aase antibody
Anti-Adenosine 5'-Monophosphoramidase Fhit antibody
Anti-Adenylylsulfatase antibody
Anti-Adenylylsulfate-Ammonia Adenylyltransferase antibody
Anti-Diadenosine 5' -5'''-P1 -P3-Triphosphate Hydrolase antibody
Anti-Dinucleosidetriphosphatase antibody
Anti-Fragile Histidine Triad Protein antibody
Anti-FHIT antibody

Information sourced from Uniprot.org

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