• Western blot analysis of extracts of various cell lines, using FGFR2 antibody (STJ11101589) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of extracts from wild type (WT) and FGFR2 knockout (KO) 293T cells, using FGFR2 antibody (STJ11101589) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-FGFR2 antibody (1-100) [S9MR] (STJ11101589)

SKU:
STJ11101589

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-FGFR2 (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FGFR2
Gene ID: 2263
Uniprot ID: FGFR2_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FGFR2 (P21802).
Immunogen Sequence: MVSWGRFICLVVVTMATLSL ARPSFSLVEDTTLEPEEPPT KYQISQPEVYVAAPGESLEV RCLLKDAAVISWTKDGVHLG PNNRTVLIGEYLQIKGATPR
Post Translational Modifications Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on several tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer. Phosphorylation at Tyr-769 is essential for interaction with PLCG1. N-glycosylated in the endoplasmic reticulum. The N-glycan chains undergo further maturation to an Endo H-resistant form in the Golgi apparatus. Ubiquitinated. FGFR2 is rapidly ubiquitinated after autophosphorylation, leading to internalization and degradation. Subject to degradation both in lysosomes and by the proteasome.
Function Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal FGFR2 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1.
Protein Name Fibroblast Growth Factor Receptor 2
Fgfr-2
K-Sam
Kgfr
Keratinocyte Growth Factor Receptor
Cd Antigen Cd332
Database Links Reactome: R-HSA-109704
Reactome: R-HSA-1257604
Reactome: R-HSA-190375
Reactome: R-HSA-190377
Reactome: R-HSA-2023837
Reactome: R-HSA-2033519
Reactome: R-HSA-2219530
Reactome: R-HSA-5654221
Reactome: R-HSA-5654695
Reactome: R-HSA-5654699
Reactome: R-HSA-5654700
Reactome: R-HSA-5654727
Reactome: R-HSA-5655253
Reactome: R-HSA-5673001
Reactome: R-HSA-6811558
Reactome: R-HSA-8851708
Reactome: R-HSA-8853333
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Golgi Apparatus
Cytoplasmic Vesicle
Detected On Osteoblast Plasma Membrane Lipid Rafts
After Ligand Binding
The Activated Receptor Is Rapidly Internalized And Degraded
Isoform 1: Cell Membrane
Isoform 3: Cell Membrane
Isoform 8: Secreted
Isoform 13: Secreted
Alternative Antibody Names Anti-Fibroblast Growth Factor Receptor 2 antibody
Anti-Fgfr-2 antibody
Anti-K-Sam antibody
Anti-Kgfr antibody
Anti-Keratinocyte Growth Factor Receptor antibody
Anti-Cd Antigen Cd332 antibody
Anti-FGFR2 antibody
Anti-BEK antibody
Anti-KGFR antibody
Anti-KSAM antibody

Information sourced from Uniprot.org

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