• Immunofluorescence analysis of PC-12 using FGF2 Rabbit monoclonal antibody (STJ11104845) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 using FGF2 Rabbit monoclonal antibody (STJ11104845) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa using FGF2 Rabbit monoclonal antibody (STJ11104845) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using FGF2 antibody (STJ11104845) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-FGF2 antibody (100-200) [S4845RM] (STJ11104845)

SKU:
STJ11104845

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Fibroblast Growth Factor 2 (100-200) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S4845RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FGF2
Gene ID: 2247
Uniprot ID: FGF2_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human FGF2 (NP_001997.5).
Immunogen Sequence: RAPERVGGRGRGRGTAAPRA APAARGSRPGPAGTMAAGSI TTLPALPEDGGSGAFPPGHF KDPKRLYCKNGGFFLRIHPD GRVDGVREKSDPHIKLQLQA E
Tissue Specificity Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
Post Translational Modifications Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion. Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
Function Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation.
Protein Name Fibroblast Growth Factor 2
Fgf-2
Basic Fibroblast Growth Factor
Bfgf
Heparin-Binding Growth Factor 2
Hbgf-2
Database Links Reactome: R-HSA-109704
Reactome: R-HSA-1257604
Reactome: R-HSA-1839122
Reactome: R-HSA-1839130
Reactome: R-HSA-190322
Reactome: R-HSA-190370
Reactome: R-HSA-190372
Reactome: R-HSA-190373
Reactome: R-HSA-190375
Reactome: R-HSA-190377
Reactome: R-HSA-2033519
Reactome: R-HSA-2219530
Reactome: R-HSA-2892247
Reactome: R-HSA-3000170
Reactome: R-HSA-3000171
Reactome: R-HSA-5654219
Reactome: R-HSA-5654221
Reactome: R-HSA-5654227
Reactome: R-HSA-5654228
Reactome: R-HSA-5654687
Reactome: R-HSA-5654688
Reactome: R-HSA-5654689
Reactome: R-HSA-5654693
Reactome: R-HSA-5654695
Reactome: R-HSA-5654699
Reactome: R-HSA-5654700
Reactome: R-HSA-5654704
Reactome: R-HSA-5654706
Reactome: R-HSA-5654710
Reactome: R-HSA-5654712
Reactome: R-HSA-5654719
Reactome: R-HSA-5654720
Reactome: R-HSA-5654726
Reactome: R-HSA-5654727
Reactome: R-HSA-5654732
Reactome: R-HSA-5654733
Reactome: R-HSA-5655253
Reactome: R-HSA-5655302
Reactome: R-HSA-5655332
Reactome: R-HSA-5658623
Reactome: R-HSA-5673001
Reactome: R-HSA-6785807
Reactome: R-HSA-6811558
Reactome: R-HSA-8851708
Reactome: R-HSA-9761174
Cellular Localisation Secreted
Nucleus
Exported From Cells By An Endoplasmic Reticulum (Er)/Golgi-Independent Mechanism
Unconventional Secretion Of Fgf2 Occurs By Direct Translocation Across The Plasma Membrane
Binding Of Exogenous Fgf2 To Fgfr Facilitates Endocytosis Followed By Translocation Of Fgf2 Across Endosomal Membrane Into The Cytosol
Nuclear Import From The Cytosol Requires The Classical Nuclear Import Machinery
Involving Proteins Kpna1 And Kpnb1
As Well As Cep57
Alternative Antibody Names Anti-Fibroblast Growth Factor 2 antibody
Anti-Fgf-2 antibody
Anti-Basic Fibroblast Growth Factor antibody
Anti-Bfgf antibody
Anti-Heparin-Binding Growth Factor 2 antibody
Anti-Hbgf-2 antibody
Anti-FGF2 antibody
Anti-FGFB antibody

Information sourced from Uniprot.org

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