• Immunofluorescence analysis of paraffin-embedded mouse testis using FER1L5 Rabbit polyclonal antibody (STJ118371) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded rat testis using FER1L5 Rabbit polyclonal antibody (STJ118371) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using FER1L5 Rabbit polyclonal antibody (STJ118371) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 150s.

Anti-FER1L5 antibody (1350-1670) (STJ118371)

SKU:
STJ118371

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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FER1L5 (1350-1670) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FER1L5
Gene ID: 90342
Uniprot ID: FR1L5_HUMAN
Immunogen Region: 1350-1670
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1350-1670 of human FER1L5 (NP_001280012.1).
Immunogen Sequence: KLPTLSEKKHQDFLGYLYRK FWFKSSKAEDEYEHEVDWWS KLFWATDEHKSLKYKYKDYH TLKVYECELEAVPAFQGLQD FCQTFKLYQEQPKLDSPVVG EFKGLFRIYPFPENPEAPKP PLQFLVWPEREDFPQPCLVR VYMVRAINLQPQDYNGLCDP YVILKLGKTELGNRDMYQPN TLDPIFGMMFELTCNIPLEK DLEIQLYDFDLFSPDDKIGT TVIDLENRLLSGFGAHCGL
Function Plays a role in myoblast fusion.probable mediator of endocytic recycling for membrane trafficking events during myotube formation.
Protein Name Fer-1-Like Protein 5
Cellular Localisation Cell Membrane
Membrane
Single-Pass Membrane Protein
Colocalizes With Ehd1 And Ehd2 At Plasma Membrane In Myoblasts And Myotubes
Localizes Into Foci At The Plasma Membrane
Alternative Antibody Names Anti-Fer-1-Like Protein 5 antibody
Anti-FER1L5 antibody

Information sourced from Uniprot.org

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